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EMAGE:844

Pitx2 paired-like homeodomain transcription factor 2 ( MGI:109340)
TS14 (9.0 dpc)
in situ hybridisation

Data Images
EMAGE:844 EMAGE:844
Figure 2D of Campione et al., 1999 [PMID:10021341] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 2C of Campione et al., 1999 [PMID:10021341] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: bw - body wall; g - gut; lsp - left splanchnopleura; rsp - right splanchnopleura; va - vitelline artery. Image D is a section taken from the embryo shown in image C at the level indicated.
Expression Pattern Description
Spatial Annotation:
EMAGE:844EMAGE:844Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
844_voxel_strong_3D_1.wlz
844_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:844_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
mesenchyme derived from somatopleure
detected detected
gradedExpression is stronger on the left side.
mesenchyme derived from splanchnopleure
detected detected
regionalExpression is present solely on the left side.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1330045
Entity Detected:Pitx2, paired-like homeodomain transcription factor 2 ( MGI:109340)
Notes:The Pitx2 probe used in this study by Campione et al., 1999 [PMID:10021341] is described as "1.7kb XhoI-EcoRI fragment corresponding to entire coding sequence plus 5' and 3' UTR." Exact end points are undefined.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:9.0 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount plus section of wholemount
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
General Information
Authors:Campione et al., 1999 [PMID:10021341] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10021341] Campione M, Steinbeisser H, Schweickert A, Deissler K, van Bebber F, Lowe LA, Nowotschin S, Viebahn C, Haffter P, Kuehn MR, Blum M 1999 The homeobox gene Pitx2: mediator of asymmetric left-right signaling in vertebrate heart and gut looping. Development (126):1225-34
Links:MGI:2148888 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI