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EMAGE:8443

Rnd2 Rho family GTPase 2 ( MGI:1338755)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8443 EMAGE:8443 EMAGE:8443 EMAGE:8443 EMAGE:8443
"Pseudo-wholemount" of euxassay_009690. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009690_01 euxassay_009690_02 euxassay_009690_03 euxassay_009690_04
EMAGE:8443 EMAGE:8443 EMAGE:8443 EMAGE:8443 EMAGE:8443
euxassay_009690_05 euxassay_009690_06 euxassay_009690_07 euxassay_009690_08 euxassay_009690_09
EMAGE:8443 EMAGE:8443 EMAGE:8443 EMAGE:8443 EMAGE:8443
euxassay_009690_10 euxassay_009690_11 euxassay_009690_12 euxassay_009690_13 euxassay_009690_14
EMAGE:8443 EMAGE:8443 EMAGE:8443 EMAGE:8443 EMAGE:8443
euxassay_009690_15 euxassay_009690_16 euxassay_009690_17 euxassay_009690_18 euxassay_009690_19
EMAGE:8443 EMAGE:8443 EMAGE:8443
euxassay_009690_20 euxassay_009690_21 euxassay_009690_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8443Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8443_wholemount_strong.wlz
8443_wholemount_moderate.wlz
8443_wholemount_weak.wlz
8443_wholemount_possible.wlz
8443_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8443_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
cerebral cortex mantle layer
strong strong
regionalstrong expression: see section 07 08 09 10 11 13 14 15 16 17 18 19 moderate expression: see section 03 04 05 06 20 21 22
not examined not examined
regionalnot examined expression: see section 03
olfactory cortex mantle layer
strong strong
regionalstrong expression: see section 08 09 10 13 14 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36973
Entity Detected:Rnd2, Rho family GTPase 2 ( MGI:1338755)
Sequence:sense strand is shown

>T36973
GCTCAACATGTGGGATACTTCAGGTTCCTCTTACTATGACAATGTCCGGCCTCTGGCCTACCCGGATTCT
GATGCTGTGCTTATCTGCTTTGACATTAGCCGGCCAGAAACACTGGACAGTGTCCTCAAGAAGTGGCAAG
GAGAGACTCAGGAGTTTTGCCCCAATGCCAAGGTGGTGCTGGTTGGCTGTAAGCTGGACATGCGGACTGA
CCTGGCCACACTGAGGGAGCTATCCAAGCAGAGACTCATCCCTGTCACACATGAGCAGGGTACTGTGCTG
GCCAAGCAAGTGGGAGCTGTGTCCTACGTTGAATGTTCCTCCCGATCTTCTGAGCGCAGTGTCCGGGATG
TCTTCCAT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 98924. Forward Primer - name:098924_F_cDNA_Rohn, sequence:GCTCAACATGTGGGATACTTCA; Reverse Primer - name:098924_N_SP6_cDNA_Rohn, sequence:ATGGAAGACATCCCGGACACT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8439 same embryo
 EMAGE:8438 same embryo
 EMAGE:8442 same embryo
 EMAGE:8440 same embryo
 EMAGE:8441 same embryo
 EurExpress:euxassay_009690 same experiment
 MGI:4827759 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS