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EMAGE:847

Hoxb3 homeobox B3 ( MGI:96184)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:847 EMAGE:847
Figure 4C of Hunt et al., 1991 [PMID:1685117] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright. Figure 4A of Hunt et al., 1991 [PMID:1685117] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Abbreviations used: b1-b3, 1st to 3rd branchial arch; nt, neural tube; ov, otic vesicle. Only the data from Fig 4C has been spatially mapped in this EMAGE entry.
Expression Pattern Description
Spatial Annotation:
EMAGE:847EMAGE:847Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
847_voxel_strong_3D_1.wlz
847_voxel_moderate_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:847_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
2nd branchial arch
not detected not detected
Hybridization is absent from the first 2 branchial arches and confined to the third branchial arch and posterior (Fig 4c).
rhombomere 05
detected detected
The cutoff of expression is shown in the neural tube at the r4/r5 boundary (Fig 4a).
3rd branchial arch
detected detected
Hybridization is absent from the first 2 branchial arches and confined to the third branchial arch and posterior (Fig4c).
neural tube
detected detected
The cutoff of expression is shown in the neural tube at the r4/r5 boundary (Fig 4a).
1st branchial arch
not detected not detected
Hybridization is absent from the first 2 branchial arches and confined to the third branchial arch and posterior (Fig 4c).
rhombomere 07
detected detected
The cutoff of expression is shown in the neural tube at the r4/r5 boundary (Fig 4a).
rhombomere 08
detected detected
The cutoff of expression is shown in the neural tube at the r4/r5 boundary (Fig 4a).
rhombomere 06
detected detected
The cutoff of expression is shown in the neural tube at the r4/r5 boundary (Fig 4a).
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1346564
Entity Detected:Hoxb3, homeobox B3 ( MGI:96184)
Notes:The probe used in this study by Hunt et al., 1991 [PMID:1685117] is indicated as that originally described by Wilkinson et al., 1989 [PMID:2571936] ie. a "700-bp BamHI-HindIII" fragment.
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Strain:CBA x C57BL
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:section
Procedures
Staining procedure:autoradiography
General Information
Authors:Hunt et al., 1991 [PMID:1685117] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:1685117] Hunt P, Wilkinson D, Krumlauf R 1991 Patterning the vertebrate head: murine Hox 2 genes mark distinct subpopulations of premigratory and migrating cranial neural crest. Development (112):43-50
 [ doi:10.1038/341405a0] [ PMID:2571936] Wilkinson DG, Bhatt S, Cook M, Boncinelli E, Krumlauf R 1989 Segmental expression of Hox-2 homoeobox-containing genes in the developing mouse hindbrain. Nature (341):405-9
Links:MGI:1928684 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI