Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:8486

Trim24 tripartite motif-containing 24 ( MGI:109275)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8486 EMAGE:8486 EMAGE:8486 EMAGE:8486 EMAGE:8486
"Pseudo-wholemount" of euxassay_009747. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009747_01 euxassay_009747_02 euxassay_009747_03 euxassay_009747_04
EMAGE:8486 EMAGE:8486 EMAGE:8486 EMAGE:8486 EMAGE:8486
euxassay_009747_05 euxassay_009747_06 euxassay_009747_07 euxassay_009747_08 euxassay_009747_09
EMAGE:8486 EMAGE:8486 EMAGE:8486 EMAGE:8486 EMAGE:8486
euxassay_009747_10 euxassay_009747_11 euxassay_009747_12 euxassay_009747_13 euxassay_009747_14
EMAGE:8486 EMAGE:8486 EMAGE:8486 EMAGE:8486 EMAGE:8486
euxassay_009747_15 euxassay_009747_16 euxassay_009747_17 euxassay_009747_18 euxassay_009747_19
EMAGE:8486 EMAGE:8486 EMAGE:8486 EMAGE:8486 EMAGE:8486
euxassay_009747_20 euxassay_009747_21 euxassay_009747_22 euxassay_009747_23 euxassay_009747_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8486Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8486_wholemount_strong.wlz
8486_wholemount_moderate.wlz
8486_wholemount_weak.wlz
8486_wholemount_possible.wlz
8486_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8486_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
olfactory cortex ventricular layer
moderate moderate
regionalmoderate expression: see section 10 11 15 16
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 08 16
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 13 14 15 16
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 08 09 10 11 14 15 16 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30582
Entity Detected:Trim24, tripartite motif-containing 24 ( MGI:109275)
Sequence:sense strand is shown

>T30582
TGCCCAGTTTGCAGTCAAGAGTGTGCTGAGAGACACATCATAGACAACTTTTTTGTGAAGGACACCACTG
AAGTTCCTAGTAGTACAGTAGAAAAGTCTAATCAGGTATGTACAAGCTGTGAAGACAATGCAGAAGCTAA
TGGGTTTTGTGTAGAGTGTGTTGAATGGCTCTGCAAGACATGTATTAGAGCTCACCAGAGGGTGAAGTTC
ACAAAAGACCACACAGTCAGGCAGAAAGAAGAAGTATCTCCAGAGGCAGTTGGGGTGACCAGTCAGCGAC
CAGTGTTTTGTCCCTTCCATAAAAAGGAGCAGTTGAAACTTTACTGTGAAACATGTGATAAACTGACCTG
TCGAGACTGCCAGCTGCTAGAACACAAAGAACACAGGTATCAATTTATAGAAGAAGCTTTTCAGAATCAA
AAAGTGATCATAGATACTCTAATCACCAAACTGATGGAAAAAACAAAATATATAAAGTATACAGGAAATC
AGATCCAAAATAGGATAATTGAAATAAATCAAAACCAAAAGCAGGTGGAACAGGATATTAAAGTTGCCAT
CTTCACATTGATGGTGGAGATAAACAAAAAAGGGAAAGCTCTGCTGCACCAGCTTGAGAGTCTTGCAAAG
GACCATCGAATGAAACTCATGCAACAACAGCAGGAAGTGGCTGGGCTTTCTAAGCAGTTAGAGCACGTCA
TGCATTTTTCTAAATGGGCTGTTTCCAGTGGCAGCAGCACAGCCTTGCTGTACAGCAAGCGGCTGATTAC
ATACAGGTTACGGCACCTTCTTCGTGCAAGGTGTGATGCTTCTCCTGTGACCAACACCACCATCCAGTTT
CACTGTGATCCTAGTTTCTGGGCTCAAAATATTATCAACTTGGGTTCTTTAGTAATCGAGGATAAAGAGA
GCCAGCCACAAATG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5698079), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59571. Forward Primer - name:059571_F_IRAV113_e01_Trim24, sequence:TGCCCAGTTTGCAGTCAA; Reverse Primer - name:059571_R_SP6_IRAV113_e01_Trim24, sequence:GCATTTGTGGCTGGCTCT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8489 same embryo
 EMAGE:8488 same embryo
 EMAGE:8487 same embryo
 EMAGE:8490 same embryo
 EMAGE:8485 same embryo
 EurExpress:euxassay_009747 same experiment
 MGI:4828910 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS