EMAGE mouse gene expression spatial database

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EMAGE:8492

Ptpn5 protein tyrosine phosphatase, non-receptor type 5 ( MGI:97807)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009726. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009726_01	euxassay_009726_02	euxassay_009726_03	euxassay_009726_04

euxassay_009726_05	euxassay_009726_06	euxassay_009726_07	euxassay_009726_08	euxassay_009726_09

euxassay_009726_10	euxassay_009726_11	euxassay_009726_12	euxassay_009726_13	euxassay_009726_14

euxassay_009726_15	euxassay_009726_16	euxassay_009726_17	euxassay_009726_18	euxassay_009726_19

euxassay_009726_20	euxassay_009726_21	euxassay_009726_22	euxassay_009726_23	euxassay_009726_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8492_wholemount_strong.wlz

8492_wholemount_moderate.wlz

8492_wholemount_weak.wlz

8492_wholemount_possible.wlz

8492_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8492_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

moderate

regional

moderate expression: see section 03 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 16 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 16 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 06 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 04 05 06 07 16 17 18

spinal cord

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 08 13 14 15 16 weak expression: see section 11 12

neural retina

weak

regional

weak expression: see section 01 02 03 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36921
Entity Detected:	Ptpn5, protein tyrosine phosphatase, non-receptor type 5 ( MGI:97807)
Sequence:	sense strand is shown >T36921 ATGCGAACAGTACCAGTTTGTGCACCACGCCATGAGCCTCTATGAGAAGCAGCTGTCCCTCCAGTCCTCA GAGTGACCACGCTCTTCCTCTGAGATCCACTGGGTACTGCCAAGCCTGAGGCTGGGTCTTTACCCCAACT ACCCAAGATCTTGCCTTGGGTCCTGGGCCTATTCCCTGGCCCTCCCCCTCATCCTCCCCTCTCTTCAGTC TGTTCCCCATCTATTGTCCTGGCCTGGTCCCTACCCCAAGCCTAGCTCTCCTACTGTATATACTGCAGAG TTGGGGAGGGCAGGGGAGGAATGTGCCAGGCCAGGCCTGGGGCCCCTGGGCCTGACCCACACCATATAGA CCTGAGGGCCTCAGTTTTAACCATGGTTCCACAGCTACTTGGTCCAAAATGTTTCCATGCCACACCCCAA GTGTCCTACCATAATGTGTTCTGTCTGTCCATCTATCACAGCTATTTGTACCAAACACTGTGTCCCCTCA GCCAGGAAAGGAGCACTGAACTAAGCTCTCACCCCTCAGCCCTCACCCACATCCCCACACCAGACTCCCA CCCTGCTCCCCAGGCAGAGATACTGACCTTCCTCTGGCCCCACTGGCTCCTCTGCAAAGGCAGAGTGCAT TTTGCTACCAGGATGTGGATGGCAGAGACCAGGCAGGGGAGCACATGATGGTTGACAGAGAGATCCCAGC TAAGACAGTTCCTGGGCAAAGATGAGTATCTCCAGTCTATGTGGCACTGGAGAGGAAGAGGACAGATATA GTGGGGAACCCCAAAGA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 74697. Forward Primer - name:074697_F_cDNA_Ptpn5, sequence:ATGCGAACAGTACCAGTTTGTG; Reverse Primer - name:074697_N_SP6_cDNA_Ptpn5, sequence:TCTTTGGGGTTCCCCACTATATC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8495 same embryo
	EMAGE:8497 same embryo
	EMAGE:8496 same embryo
	EMAGE:8493 same embryo
	EMAGE:8494 same embryo
	EurExpress:euxassay_009726 same experiment
	MGI:4827521 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source