EMAGE mouse gene expression spatial database

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EMAGE:8495

Hdgfl1 hepatoma derived growth factor-like 1 ( MGI:1194493)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009706. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009706_01	euxassay_009706_02	euxassay_009706_03	euxassay_009706_04

euxassay_009706_05	euxassay_009706_06	euxassay_009706_07	euxassay_009706_08	euxassay_009706_09

euxassay_009706_10	euxassay_009706_11	euxassay_009706_12	euxassay_009706_13	euxassay_009706_14

euxassay_009706_15	euxassay_009706_16	euxassay_009706_17	euxassay_009706_18	euxassay_009706_19

euxassay_009706_20	euxassay_009706_21	euxassay_009706_22	euxassay_009706_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8495_wholemount_strong.wlz

8495_wholemount_moderate.wlz

8495_wholemount_weak.wlz

8495_wholemount_possible.wlz

8495_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8495_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 04 05 06 07 08 09 10 11 12 13 14 15 16 17 20 21 22 23 weak expression: see section 02 03 18 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 04

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 16 20 weak expression: see section 02 03 07 15 17 18 19

vagus x ganglion

moderate

regional

moderate expression: see section 05 weak expression: see section 15

spinal cord

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13

dorsal root ganglion

moderate

regional

moderate expression: see section 04 05 06 07 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36929
Entity Detected:	Hdgfl1, hepatoma derived growth factor-like 1 ( MGI:1194493)
Sequence:	sense strand is shown >T36929 TCCCTTATGAGGAGTCCAAAGAGAAGTTCGGCAAGCCCAACAAGAGGCGCGGCTTCAGCGAGGGGCTGTG GGAGATCGAGCACGACCCTATGGTTGAGGCCTCCTCTAGCCTGTGCTCAGAAGAGGATCAGAGCTACACA GAGGATCCTGGGCTAGCAGAGGAGCCGGAACTAGGACAGGAGCTGGTGCAGGAGCTGGAGCCCGAATTCA TGCCTGAGCTGGAGGCAGAACCTGAGATGCCTGAGACCGAGTGTGAACAGGAGCCCGAGCCCCAGCCTGC CTATGACCTGCTGGACGCCGTGGAGGAGCCGGGCCTCACCAAGGCCGAGCCAGGAGATCAGCAAGCCGAG CATGTGCAAGAGAAGCACCCTGAGGTGGAGGCAGAGGCTGAGGCTGAGGCTGAGGCCGAGGCAGAGGCGG AGGCGGAGGCCAAGGCTGAGGTGGAGGAGCCGGGGAGTCTGAAGAGGAGCGCGGAGGATGAAGAGCCTCA TTGTCCTCTCAAACGGCCCAGGGAGGCGGCTCCTGGTGCGTTGGAGATGGAGCCTGCTGAAGAGCGCGAG GCTGAGGCCTCCCCCTTCGTGGAGGAGCCTGACCAAGCCCAGGAGCAGCTGCCTCCGTTGGAGGAAGAGG CCACAGAAAAGGCGGTCCAGGGCCTGATTGTTGGAGAAATAGAAGGCCTGTAGTCACGGTGTTTGTAGAA GAGCCCCTTTTACCCGTTCCTGCTGCCACCTGGCTGTGGCTTGGGAAACCCACTAGGGCCCAGTCTTCAA CCCAGTCCACCTCTCCTTCATTCATTCGCCTTCTACAAGCCTAAATTCCTGGCATGAGGGGACAGGCCAG TTGACATCAACTTTGTGGCTTAAGTAGTGACCACCCTTCCCACCCTAAACTCTTAGGGGCATTTTGGACC TCTGCCTAGGAACCAGGAGTGAAGAGGGATGGAAGATGAAGAGCAGGGACA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 97859. Forward Primer - name:097859_F_cDNA_Pwwp1, sequence:TCCCTTATGAGGAGTCCAAAGA; Reverse Primer - name:097859_N_SP6_cDNA_Pwwp1, sequence:TGTCCCTGCTCTTCATCTTCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8497 same embryo
	EMAGE:8496 same embryo
	EMAGE:8493 same embryo
	EMAGE:8494 same embryo
	EMAGE:8492 same embryo
	EurExpress:euxassay_009706 same experiment
	MGI:4825347 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source