EMAGE mouse gene expression spatial database

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EMAGE:8498

Fam122b family with sequence similarity 122, member B ( MGI:1926005)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002251. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002251_01	euxassay_002251_02	euxassay_002251_03	euxassay_002251_04

euxassay_002251_05	euxassay_002251_06	euxassay_002251_07	euxassay_002251_08	euxassay_002251_09

euxassay_002251_10	euxassay_002251_11	euxassay_002251_12	euxassay_002251_13	euxassay_002251_14

euxassay_002251_15	euxassay_002251_16	euxassay_002251_17	euxassay_002251_18	euxassay_002251_19

euxassay_002251_20	euxassay_002251_21	euxassay_002251_22	euxassay_002251_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8498_wholemount_strong.wlz

8498_wholemount_moderate.wlz

8498_wholemount_weak.wlz

8498_wholemount_possible.wlz

8498_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8498_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand mesenchyme

strong

regional

strong expression: see section 01

hindlimb digit 1 mesenchyme

strong

regional

strong expression: see section 08 09 moderate expression: see section 22 23

hindlimb digit 2 mesenchyme

strong

regional

strong expression: see section 07 08 09 10 moderate expression: see section 22 23 weak expression: see section 21

hindlimb digit 3 mesenchyme

strong

regional

strong expression: see section 06 07 08 10 moderate expression: see section 22 23 weak expression: see section 21

hindlimb digit 4 mesenchyme

strong

regional

strong expression: see section 08 10 moderate expression: see section 22 23 weak expression: see section 21

hindlimb digit 5 mesenchyme

strong

regional

strong expression: see section 09 10 moderate expression: see section 22 23

trachea

strong

regional

strong expression: see section 13 14

axial skeleton

strong

regional

strong expression: see section 08 09 10 11 12 13 14 16 moderate expression: see section 17 18

cranium

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

pectoral girdle and thoracic body wall skeleton

strong

regional

strong expression: see section 12 13 14 15 16 moderate expression: see section 05 06 07 08 11 weak expression: see section 09 10 17 18 19

axial skeleton tail region

strong

regional

strong expression: see section 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2079
Entity Detected:	Fam122b, family with sequence similarity 122, member B ( MGI:1926005)
Sequence:	sense strand is shown >T2079 TGGCCTCGAGCCAGATTCGGCACGAGGTCTTGCCCCCTACCCTCCTGCATCTCTGTCCCAACCATCAGTC ACCACTTCTTCTTACAGATTCCCTTATCACATTCTTGTCTTTTTCGTTGTAAAGTAACCTTTTATTATTG TTGTCTTCTATATTGTTTAAATGGACTAGAAAGCTAAGTTTGTGCAGGGATTGTTTTGAAATAAATAAAA CTGGTAATATGCTAATCCACAGAGCAGCTTTGTTGACAGTACCCCTTTATTTTTATATAAAGTCTAATAT TTGAAATGTAGTTCTTGTTATAAAGTAAAATTCTTTCTTCCTATTCTCATATTATATCATATATTTTCAG CTTCTCTTTGAAAACTACTACAAAAAAATGAAACCATAATAAAATCCTATAGATACTGGTTTTGCTTGAC TGACTTATAAATCACTGTTTTATGATAACTGCTTTTGGAATAATAGAAAGTTTTGTGAAATGTTGACCTA GTGTATACCCTAAAATTGTGAATTTAATAAAGTATATATACGTGTATAAAAAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:846350 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:846350 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4824699 same experiment
	EurExpress:euxassay_002251 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source