EMAGE mouse gene expression spatial database

EMAGE:850

Igf2 insulin-like growth factor 2 ( MGI:96434)
TS12 (8.0 dpc)
in situ hybridisation

Data Images


Fig 2J Lee et al, 1990 [PMID:1964408] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

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Notes:

Image annotations: nf, neural folds; h, heart; fg, foregut; vf, ventral foregut; df, dorsal foregut; se, surface ectoderm.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

3D mapping

3D context movie

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foregut diverticulum endoderm

strong

regional

The hybridization signal was localized in the ventral and lateral walls, whereas the dorsal wall was practically unlabeled at this stage

heart

strong

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:1344322
Entity Detected:	Igf2, insulin-like growth factor 2 ( MGI:96434)
Sequence:	sense strand is shown >MGI:1344322 GGATCCCAGTGGGGAAGTCGATGTTGGTGCTTCTCATCTCTTTGGCCTTCGCCTTGTGCTGCATCGCTGC TTACCGCCCCAGCGAGACTCTGTGCGGAGGGGAGCTTGTTGACACGCTTCAGTTTGTCTGTTCGGACCGC GGCTTCTACTTCAGCAGGCCTTCAAGCCGTGCCAACCGTCGCAGCCGTGGCATCGTGGAAGAGTGCTGCT TCCGCAGCTGCGACTTGGCCCTCCTGGAGACATACTGTGCCACCCCCGCCAAGTCCGAGAGGGACGTGTC TACCTCTCAGGCCGTACTTCCGGACGACTTCCCCAGATACCCCGTGGGCAAGTTCTTCAAATTCGACACC TGGAGACAGTCCGCGGGACGCCTGCGCAGAGGCCTGCCTGCCCTCCTGCGTGCCCGCCGGGGTCGCATGC TTGCCAAAGAGCTCGAAGCGTTCAGAGAGGCCAAGCGCCACCGTCCCCTGATCGTGTTACCACCCAAAGA CCCCGCCCACGGGGGAGCCTCTTCGGAGATGTCCAGCAACCATCAGTGAACCAAAT
	nt 1136 - nt 1681 of NM_031511.1
Notes:	The Igf2 probe used in this study by Lee et al, 1990 [PMID:1964408] is denoted as the rat probe used by Stylianopoulou et al, 1988 [PMID:3246220] . The authors note that the rat and mouse sequences differ over this region by 10 out of 545 nt. Stylianopoulou et al in turn define the probe as "a 545-bp fragment from the rat IGF-II cDNA clone 27 (Soares et al, 1986 [PMID:2438416] ). This fragment extends between a BamHI site, located one nucleotide downstream from the ATG initiator, and an EcoRI linker present at the end of the fragment, three nucleotides downstream from the TGA terminator".
Chemistry:	RNA
Strand:	antisense
Label:	S35

Specimen

Procedures

Staining procedure:

autoradiography

General Information

Authors:	Lee et al, 1990 [PMID:1964408] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ PMID:1964408] Lee JE, Pintar J, Efstratiadis A 1990 Pattern of the insulin-like growth factor II gene expression during early mouse embryogenesis. Development (110):151-9
	[ PMID:3246220] Stylianopoulou F, Efstratiadis A, Herbert J, Pintar J 1988 Pattern of the insulin-like growth factor II gene expression during rat embryogenesis. Development (103):497-506
	[ doi:10.1016/0022-2836(86)90025-2] [ PMID:2438416] Soares MB, Turken A, Ishii D, Mills L, Episkopou V, Cotter S, Zeitlin S, Efstratiadis A 1986 Rat insulin-like growth factor II gene. A single gene with two promoters expressing a multitranscript family. J Mol Biol (192):737-52
Links:	MGI:1344456 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source