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EMAGE:8517

Chd8 chromodomain helicase DNA binding protein 8 ( MGI:1915022)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8517 EMAGE:8517 EMAGE:8517 EMAGE:8517 EMAGE:8517
"Pseudo-wholemount" of euxassay_009737. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009737_01 euxassay_009737_02 euxassay_009737_03 euxassay_009737_04
EMAGE:8517 EMAGE:8517 EMAGE:8517 EMAGE:8517 EMAGE:8517
euxassay_009737_05 euxassay_009737_06 euxassay_009737_07 euxassay_009737_08 euxassay_009737_09
EMAGE:8517 EMAGE:8517 EMAGE:8517 EMAGE:8517 EMAGE:8517
euxassay_009737_10 euxassay_009737_11 euxassay_009737_12 euxassay_009737_13 euxassay_009737_14
EMAGE:8517 EMAGE:8517 EMAGE:8517 EMAGE:8517 EMAGE:8517
euxassay_009737_15 euxassay_009737_16 euxassay_009737_17 euxassay_009737_18 euxassay_009737_19
EMAGE:8517 EMAGE:8517 EMAGE:8517 EMAGE:8517
euxassay_009737_20 euxassay_009737_21 euxassay_009737_22 euxassay_009737_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8517Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8517_wholemount_strong.wlz
8517_wholemount_moderate.wlz
8517_wholemount_weak.wlz
8517_wholemount_possible.wlz
8517_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8517_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30602
Entity Detected:Chd8, chromodomain helicase DNA binding protein 8 ( MGI:1915022)
Sequence:sense strand is shown

>T30602
GACATGACCGGCATCACACCTATGGGCGCACTGACTGCTTCCGGGTAGAAAAGCATCTCCTGGTATATGG
TTGGGGACGTTGGAGAGATATTTTGTCTCACGGACGATTTAAGCGGCGCATGACTGAAAGAGATGTTGAG
ACAATATGCCGTGCCATCCTCGTGTACTGTCTTCTACACTACCGTGGGGATGAAAACATCAAAAGCTTCA
TTTGGGACTTGATTAGCCCTGCTGAAAATGGCAAGACAAAAGAATTGCAGAATCATTCAGGTCTGTCTAT
CCCTGTGCCCCGTGGGCGTAAGGGGAAAAAAGTCAAGTCACAAAGTACTTTTGACATTCATAAGGCAGAC
TGGATCCGGAAATACAACCCTGATACTCTGTTCCAAGATGAGAGTTATAAGAAGCATTTGAAACATCAGT
GTAACAAGGTACTGTTGAGAGTCCGGATGCTCTACTACCTGAGACAGGAGGTTATTGGAGACCAGGCAGA
GAAGGTGTTAGGAGGTGCCATAGCCAGTGAGATTGACATATGGTTCCCAGTGGTGGATCAGCTGGAGGTT
CCTACAACTTGGTGGGATAGTGAGGCTGACAAATCCCTGCTCATTGGCGTTTTTAAGCATGGCTATGAGA
AATACAATACCATGAGGGCAGACCCTGCCTTGTGCTTCCTAGAAAAGGCTGGCAGGCCAGATGACAAAGC
CATCGCAGCAGAACATAGAGTGTTGGATAATTTCTCCGACCTGGTAGAAGGGATTGACTTTGATAAGGAT
TGTGAAGATCCTGAATATAAACCCCTCCAGGGTCCTCCAAAGGACCCAGATGATGAGGGTGATCCCTTGA
TGATGATGGATGAGGAGATCTCAGTCATCGACGGAGAGGAAGCCCAGGTAACTCAACAGCCAGGGCATTT
ATTCTGGCCTCCAGGCTCCGCCCTCACAGCTAGGCTTCGCCGCCTAGTAACAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5721164), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59922. Forward Primer - name:059922_F_IRAV114_d05_Chd8, sequence:GACATGACCGGCATCACA; Reverse Primer - name:059922_R_SP6_IRAV114_d05_Chd8, sequence:GCTGTTACTAGGCGGCGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8515 same embryo
 EMAGE:8514 same embryo
 EMAGE:8518 same embryo
 EMAGE:8516 same embryo
 EurExpress:euxassay_009737 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS