EMAGE mouse gene expression spatial database

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EMAGE:8520

Prss23 protease, serine, 23 ( MGI:1923703)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007251. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007251_01	euxassay_007251_02	euxassay_007251_03	euxassay_007251_04

euxassay_007251_05	euxassay_007251_06	euxassay_007251_07	euxassay_007251_08	euxassay_007251_09

euxassay_007251_10	euxassay_007251_11	euxassay_007251_12	euxassay_007251_13	euxassay_007251_14

euxassay_007251_15	euxassay_007251_16	euxassay_007251_17	euxassay_007251_18	euxassay_007251_19

euxassay_007251_20	euxassay_007251_21	euxassay_007251_22	euxassay_007251_23	euxassay_007251_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8520_wholemount_strong.wlz

8520_wholemount_moderate.wlz

8520_wholemount_weak.wlz

8520_wholemount_possible.wlz

8520_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8520_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

physiological umbilical hernia

moderate

regional

moderate expression: see section 16

viscerocranium

moderate

regional

Expression in the turbinate bone.

lower jaw incisor

moderate

regional

moderate expression: see section 11 12 15 16

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 15 16

metanephros

weak

regional

weak expression: see section 08 09 10 11 18 19 20

axial skeleton

moderate

regional

moderate expression: see section 14 15 16

axial skeleton tail region

moderate

regional

moderate expression: see section 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1810
Entity Detected:	Prss23, protease, serine, 23 ( MGI:1923703)
Sequence:	sense strand is shown >T1810 ATTCGGNACGAGGCTCCAGCGAAGCAGCTCCCAGGGGGCAGGATCCACTTCTCTGGTTATGACAATGACC GGCCCGGCAATTTGGTGTACCGCTTCTGTGATGTCAAAGATGAGACCTACGACCTTCTCTACCAGCAGTG TGACGCCCAGCCCGGGGCCAGTGGTTCAGGGGTCTATGTGAGGATGTGGAAGAGACCACAGCAGAAATGG GAAAGAAAAATTATCGGCATCTTTTCAGGGCACCAGTGGGTGGACATGAATGGCTCTCCACAGGATTTCA ACGTGGCAGTTAGAATCACGCCTCTTAAATATGCCCAGATTTGCTATTGGATTAAAGGAAACTACCTAGA TTGCAGGGAGGGGTGACATGCGTCTTCTTGCCAGCACCAATGGTCTTTTTGCACTCATTGTAGGAGAGGC TAGCTTTTTATCATTGACTCTTGTGGTGTGAGTCACATAGTATCTTTTACCTAGTATTCTTCAAATGGCA AAAATTATTGGCTATATTATTTTAAAACTGTTGTGTGCGTTATAG
Notes:	The probe template was PCR amplified from IMAGE:576361 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:576361 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8523 same embryo
	EMAGE:8519 same embryo
	EMAGE:8521 same embryo
	EMAGE:8522 same embryo
	EurExpress:euxassay_007251 same experiment
	MGI:4827456 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source