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EMAGE:8542

Bfsp1 beaded filament structural protein 1, in lens-CP94 ( MGI:101770)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8542 EMAGE:8542 EMAGE:8542 EMAGE:8542 EMAGE:8542
"Pseudo-wholemount" of euxassay_009902. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009902_01 euxassay_009902_02 euxassay_009902_03 euxassay_009902_04
EMAGE:8542 EMAGE:8542 EMAGE:8542 EMAGE:8542 EMAGE:8542
euxassay_009902_05 euxassay_009902_06 euxassay_009902_07 euxassay_009902_08 euxassay_009902_09
EMAGE:8542 EMAGE:8542 EMAGE:8542 EMAGE:8542 EMAGE:8542
euxassay_009902_10 euxassay_009902_11 euxassay_009902_12 euxassay_009902_13 euxassay_009902_14
EMAGE:8542 EMAGE:8542 EMAGE:8542 EMAGE:8542 EMAGE:8542
euxassay_009902_15 euxassay_009902_16 euxassay_009902_17 euxassay_009902_18 euxassay_009902_19
EMAGE:8542 EMAGE:8542 EMAGE:8542 EMAGE:8542
euxassay_009902_20 euxassay_009902_21 euxassay_009902_22 euxassay_009902_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8542Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8542_wholemount_strong.wlz
8542_wholemount_moderate.wlz
8542_wholemount_weak.wlz
8542_wholemount_possible.wlz
8542_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8542_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
lens
strong strong
regionalstrong expression: see section 01
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30651
Entity Detected:Bfsp1, beaded filament structural protein 1, in lens-CP94 ( MGI:101770)
Sequence:sense strand is shown

>T30651
TCTCTGATCACCCCGAGCCATGGGGCCCCTCTCAGCCTTGGATCCAGTGTGAAAGATCTTGCCAGGGCCG
TGCAGGATATTACAGCAGCTAAACCGAGACAGAAGGCCCTCCCCAAGAGCCTTCCAAAAAGAAAGGAGAT
TATAGCTCAAGATAAGGTGGAGGAAACTCTGGAAGATGCACCATTAAAACCTCCGCAGGAGCCAAAGGCA
CTGCAGGTGGAACGGAAAGCAGAAGGTGGCTCTCAGCCCGGAGCAGGGGGAGGACATGGAGTAAGTCCCA
CACAAGAAGGGGGTCCTGAGGATGTGCCAGACGGGGGCCAGATAAGCAAAGCCTTTGGGAAACTGTGCAA
AGTAGTGAAAGAGAGAGTGAGCGGCCACAAAGAGCCCGAGCCTGAGCCGCCCACGGACCTTTTCACCAAA
GGACGGCATGTACTCGTCACAGGGGAGTCTAGTTTTGTTGACCCCGAGTTCTACTCCTCCTCCATCCCAG
CTAGAGGTGGGGTGGTGATTTCCATTGAGGAAGACTCTATGCATCATGACGGCCACGTAGAGCCCTCTCC
TGGACAGCCCATGCCACCTGTGGAGAATGGACAGGGCGTTCCCCAGGGCAGGGAAGGTGACCACTCAAAC
CATCAGCAGGGTACAGACAAGAATGGGTTACGGGCCAAGGAACCAAAAGACCTGGAGGAAAAAGATGATG
ATGGCAAGAAGGAAGCTGAGGGGAGCAGGAGACCCTGTCCTGTGATCATACCTGGTCCTGATGAACCATC
TACATCCCATTCACAGACGTCTGGGTCCAATCAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6412634), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59737. Forward Primer - name:059737_F_IRAV116_f03_Bfsp1, sequence:TCTCTGATCACCCCGAGC; Reverse Primer - name:059737_R_SP6_IRAV116_f03_Bfsp1, sequence:CCTGATTGGACCCAGACG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8540 same embryo
 EMAGE:8539 same embryo
 EMAGE:8541 same embryo
 EMAGE:8543 same embryo
 EMAGE:8544 same embryo
 EurExpress:euxassay_009902 same experiment
 MGI:4823462 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS