EMAGE mouse gene expression spatial database

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EMAGE:8544

Mpped1 metallophosphoesterase domain containing 1 ( MGI:106316)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009802. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009802_01	euxassay_009802_02	euxassay_009802_03	euxassay_009802_04

euxassay_009802_05	euxassay_009802_06	euxassay_009802_07	euxassay_009802_08	euxassay_009802_09

euxassay_009802_10	euxassay_009802_11	euxassay_009802_12	euxassay_009802_13	euxassay_009802_14

euxassay_009802_15	euxassay_009802_16	euxassay_009802_17	euxassay_009802_18	euxassay_009802_19

euxassay_009802_20	euxassay_009802_21	euxassay_009802_22	euxassay_009802_23	euxassay_009802_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8544_wholemount_strong.wlz

8544_wholemount_moderate.wlz

8544_wholemount_weak.wlz

8544_wholemount_possible.wlz

8544_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8544_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 19 20 21 22 23 24

telencephalon marginal layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

lower jaw incisor

strong

regional

strong expression: see section 11 12 15 16

lower jaw molar

strong

regional

strong expression: see section 08 18 19

upper jaw incisor

strong

regional

strong expression: see section 11 12 15 16

upper jaw molar

strong

regional

strong expression: see section 08 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30649
Entity Detected:	Mpped1, metallophosphoesterase domain containing 1 ( MGI:106316)
Sequence:	sense strand is shown >T30649 GACCAGCCAGCAACGAGTAGAATCCTTGGGATAAAAGAAATCGCCTTTGTCAGCTTTCTGCCGCCTGCTG CTGGGACCTTGAACAGTCCCATCAGGGCCACCAGGCTCATCTCTGTGTTCTGTGTGTGCATCTGCCCCTT TTTTCTAGAGCCTAACACACTCACAGCCCTGTCTCACTTGGGAAATGACAGAAGCTCGGTTCGAAAGACA CCCTCCGGCTTTGAGAAGCCGCTGCTCTCGGATGGAATTTGGGAAGTTTTATACAAGATGTCTAGCTACC CAAGTGTGTGGCTTGTGAGCCCCAAGGTTGCGGAAGTGTATGGCCTAGGCTCGGGCAGCTCTTCTCTGGC TGGAGGAAAGGAGATAGACTTGTTCCTTACGTGCATGGGTCTTGGGAATGTTCAGGGACATCTCAGGAAT TCCGACAGCCCCCGGCCAGGCCTAGAGCAGATGGCTTGGCTTAGTCCCCCATTCCTTCCTCAGGACCCTG CTGCCAATGTGGACAATGGCTGAGTGTCTCCAGCTTCTGGCTCAGAACCTGGATTTGGGGAGGGGAGCTT TGTTCTTCTGGGTGGCCAGAACCTGCTGGTTACTCATCACAGTGAGGCCATCTTAGATCATCTGGGACCA CACTACTTTGTAGATCACGATGGCCAGGGGATTCTGTGGGCTGAGACCTATGCCTGGTCATGTACATGCC CCATGTCTGTGGCATGTGTGTGCTTCTCCAAACCTAACCAGGTTGTTAGCATCTACAACGAGCTCCATCC TCCATGTGATTGATTTTTCCTTCCGAGGGTTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6810744), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59497. Forward Primer - name:059497_F_IRAV116_e03_D15Bwg0669e, sequence:GACCAGCCAGCAACGAGT; Reverse Primer - name:059497_R_SP6_IRAV116_e03_D15Bwg0669e, sequence:GGAACCCTCGGAAGGAAA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8540 same embryo
	EMAGE:8539 same embryo
	EMAGE:8541 same embryo
	EMAGE:8543 same embryo
	EMAGE:8542 same embryo
	EurExpress:euxassay_009802 same experiment
	MGI:4826432 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source