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EMAGE:856

Pitx2 paired-like homeodomain transcription factor 2 ( MGI:109340)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:856 EMAGE:856 EMAGE:856
Figure 4F of Campione et al., 1999 [PMID:10021341] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 4D of Campione et al., 1999 [PMID:10021341] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 4E of Campione et al., 1999 [PMID:10021341] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image E is a left side view and Image D is a ventral view of the heart shown in Image F. Image annotations: at - atrium; ot - outflow tract; v - ventricle.
Expression Pattern Description
Spatial Annotation:
EMAGE:856Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
856_wholemount_strong_3D_1.wlz
856_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:856_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
primitive ventricle
moderate moderate
regionalExpression is confined to the left side but is less pronounced than in the atrium.
outflow tract
moderate moderate
regionalExpression is confined to the left side but is less pronounced than in the atrium.
heart
detected detected
regionalExpression is predominantly on the left side.
heart atrium
strong strong
regionalExpression is predominantly on the left side.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1330045
Entity Detected:Pitx2, paired-like homeodomain transcription factor 2 ( MGI:109340)
Notes:The Pitx2 probe used in this study by Campione et al., 1999 [PMID:10021341] is described as "1.7kb XhoI-EcoRI fragment corresponding to entire coding sequence plus 5' and 3' UTR." Exact end points are not defined by the authors (EcoRI and XhoI are linker derived).
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Campione et al., 1999 [PMID:10021341] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10021341] Campione M, Steinbeisser H, Schweickert A, Deissler K, van Bebber F, Lowe LA, Nowotschin S, Viebahn C, Haffter P, Kuehn MR, Blum M 1999 The homeobox gene Pitx2: mediator of asymmetric left-right signaling in vertebrate heart and gut looping. Development (126):1225-34
Links:MGI:2148888 same experiment
 EMAGE:857 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI