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EMAGE:8591

Gm12295 predicted gene 12295 ( MGI:3650535)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8591 EMAGE:8591 EMAGE:8591 EMAGE:8591 EMAGE:8591
"Pseudo-wholemount" of euxassay_016021. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016021_02 euxassay_016021_03 euxassay_016021_04 euxassay_016021_05
EMAGE:8591 EMAGE:8591 EMAGE:8591 EMAGE:8591 EMAGE:8591
euxassay_016021_07 euxassay_016021_08 euxassay_016021_09 euxassay_016021_10 euxassay_016021_12
EMAGE:8591 EMAGE:8591 EMAGE:8591 EMAGE:8591 EMAGE:8591
euxassay_016021_13 euxassay_016021_14 euxassay_016021_15 euxassay_016021_16 euxassay_016021_17
EMAGE:8591 EMAGE:8591 EMAGE:8591 EMAGE:8591 EMAGE:8591
euxassay_016021_18 euxassay_016021_19 euxassay_016021_20 euxassay_016021_21 euxassay_016021_22
EMAGE:8591 EMAGE:8591 EMAGE:8591 EMAGE:8591
euxassay_016021_23 euxassay_016021_24 euxassay_016021_25 euxassay_016021_26

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8591Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8591_wholemount_strong.wlz
8591_wholemount_moderate.wlz
8591_wholemount_weak.wlz
8591_wholemount_possible.wlz
8591_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8591_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
esophagus
weak weak
regionalweak expression: see section 11 12
lung
weak weak
regionalweak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39731
Entity Detected:Gm12295, predicted gene 12295 ( MGI:3650535)
Sequence:sense strand is shown

>T39731
CCCGCCTCTAAGAAGAACATAAGCTTTTTGCACAGTATTCTCTCAGGAGCTTTTATTGAGACTCATAAAG
GAAGCAACAACACTTGAGAGTGTTGCAAAGTGGAAAAAACAAAACAGAAACAAAAACAACAACAAAAAAC
ACGTGAACATGACGTTGGGGGAAAGGATGAAACCAGCCATCGCAAGCCAGGAGAATCCTTGGGAAGTGAG
GCTCAGCGGCTGAGCAGCCTGAGCACAGAGGTTCAGGGTCAGGGTAGGGTGCCTAGCGAATTCCCGTAAC
GCAGGACACTGCCCAAGCCAGCTTCATAAAAAGTCACTCCGTTGATTTCAGGCTCAATGGGAACATCTCT
TGGCTGTTTGCTGAATTTAGACACTTTAAAAAGTTTTCTTTGACTGAACAAGGGGTTCCTGAGACCAGCT
TCCTGAGGGTATCTTGGTAAACAAGCCTCCCACCACGAATTTCCTTGCTGTTATTGTAACTGGTTTTAAC
TGCACTTGAGTTTGTTTGGTTTGGTCTTTTGTTGTTTTTTTGTTTTTGTTTTTTTTCTTTTTTCCTTTTC
AGACAGTTGCACTTAAAATGTACTAACTTTTCAATGAGAGATGTAGGTTTGAGAGAAAATGTTTGAACTC
CCTTGATGGATCCTGTTGGTCCTAAGGCAGATCCCCTTCCCAGACAGTGTCGCTTT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 112127. Forward Primer - name:112127_F_cDNA_A730013G04, sequence:CCCGCCTCTAAGAAGAACATAA; Reverse Primer - name:112127_N_SP6_cDNA_A730013G04, sequence:AAAGCGACACTGTCTGGGAAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8593 same embryo
 EMAGE:8590 same embryo
 EMAGE:8592 same embryo
 EMAGE:8588 same embryo
 EMAGE:8589 same embryo
 EurExpress:euxassay_016021 same experiment
 MGI:4825086 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS