EMAGE mouse gene expression spatial database

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EMAGE:8617

Agr2 anterior gradient 2 (Xenopus laevis) ( MGI:1344405)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_009818_01	euxassay_009818_02	euxassay_009818_03	euxassay_009818_04	euxassay_009818_05

euxassay_009818_06	euxassay_009818_07	euxassay_009818_08	euxassay_009818_09	euxassay_009818_10

euxassay_009818_11	euxassay_009818_12	euxassay_009818_13	euxassay_009818_14	euxassay_009818_15

euxassay_009818_16	euxassay_009818_17	euxassay_009818_18	euxassay_009818_19	euxassay_009818_20

euxassay_009818_21	euxassay_009818_22	euxassay_009818_23	euxassay_009818_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8617_wholemount_strong.wlz

8617_wholemount_moderate.wlz

8617_wholemount_weak.wlz

8617_wholemount_possible.wlz

8617_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8617_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

stomach

strong

regional

strong expression: see section 05 06 07 08 09 10 11

hindgut

strong

regional

strong expression: see section 13 14

rectum

strong

regional

strong expression: see section 13 14

midgut

strong

regional

strong expression: see section 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30871
Entity Detected:	Agr2, anterior gradient 2 (Xenopus laevis) ( MGI:1344405)
Sequence:	sense strand is shown >T30871 CTTGGACGGCAACCCTTGCGGCTCACACAAAGCAGGAGGGTGGGAAGCCCAGATTTGCCATGGAGAAATT TTCAGTGTCTGCAATCCTGCTTCTTGTGGCCATTTCTGGTACCTTGGCCAAAGACACCACAGTCAAATCT GGAGCCAAAAAGGACCCAAAGGACTCTCGGCCCAAACTACCTCAGACACTCTCCAGAGGTTGGGGCGATC AGCTCATCTGGACTCAGACATACGAAGAAGCTTTATACAGATCCAAGACAAGCAACAGACCCTTGATGGT CATTCATCACTTGGACGAATGCCCACACAGTCAAGCCTTAAAGAAAGTGTTTGCTGAACATAAAGAAATC CAGAAATTGGCAGAGCAGTTTGTTCTCCTCAACCTGGTCTATGAAACAACCGACAAGCACCTTTCTCCTG ATGGCCAGTACGTCCCCAGAATTGTGTTTGTAGACCCATCCCTGACGGTGAGGGCAGACATCACTGGACG ATACTCAAACCGGCTCTACGCTTATGAACCTTCTGACACAGCTTTGTTGTACGACAACATGAAGAAAGCT CTCAAGCTGCTAAAGACAGAATTGTAGAGCTAACTGCGCACCGGGTCAGGAGACCAGAAGGCAGAAGCAC TGTGGACTTGCAGATTACAGTACAGTTTAATGTTACAACAGATATATTTTTTAAACACCCACAGGTGGGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4218549), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 16770. Forward Primer - name:016770_F_IRAV24-27_E05_Agr2, sequence:CTTGGACGGCAACCCTTG; Reverse Primer - name:016770_R_SP6_IRAV24-27_E05_Agr2, sequence:TCCCCACCTGTGGGTGTT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8616 same embryo
	EMAGE:8613 same embryo
	EMAGE:8612 same embryo
	EMAGE:8615 same embryo
	EMAGE:8614 same embryo
	EurExpress:euxassay_009818 same experiment
	MGI:4823033 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source