EMAGE mouse gene expression spatial database

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EMAGE:8621

Cebpa CCAAT/enhancer binding protein (C/EBP), alpha ( MGI:99480)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009855. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009855_01	euxassay_009855_02	euxassay_009855_03	euxassay_009855_04

euxassay_009855_05	euxassay_009855_06	euxassay_009855_07	euxassay_009855_08	euxassay_009855_09

euxassay_009855_10	euxassay_009855_11	euxassay_009855_12	euxassay_009855_13	euxassay_009855_14

euxassay_009855_15	euxassay_009855_16	euxassay_009855_17	euxassay_009855_18	euxassay_009855_19

euxassay_009855_20	euxassay_009855_21	euxassay_009855_22	euxassay_009855_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8621_wholemount_strong.wlz

8621_wholemount_moderate.wlz

8621_wholemount_weak.wlz

8621_wholemount_possible.wlz

8621_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8621_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 16 17 18 19 20

submandibular gland primordium

moderate

regional

moderate expression: see section 05 06 07 14 15 16 17

epidermis

moderate

regional

moderate expression: see section 07 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 weak expression: see section 01 02 03 04 05 06

vibrissa

weak

regional

weak expression: see section 03 04 05 06 17 18 19 20 21

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 08 09 14 15

pharyngo-tympanic tube

weak

regional

weak expression: see section 01 02 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30903
Entity Detected:	Cebpa, CCAAT/enhancer binding protein (C/EBP), alpha ( MGI:99480)
Sequence:	sense strand is shown >T30903 GCGCAAGAGCCGAGATAAAGCCAAACAACGCAACGTGGAGACGCAACAGAAGGTGCTGGAGTTGACCAGT GACAATGACCGCCTGCGCAAGCGGGTGGAACAGCTGAGCCGTGAACTGGACACGCTGCGGGGCATCTTCC GCCAGCTGCCTGAGAGCTCCTTGGTCAAGGCCATGGGCAACTGCGCGTGAGGCGCGCGGCTGCGGGACCG CCTTGGGCCGGCCCCCTGGCTGGAGACCCAGAGGATGGTTTCGGGTCGCTGGATCTCTAGGCTGCCCGGG CCGCGCAAGCCAGGACTAGGAGATTCCGGTGTGGCCTGAAAGCCTGGCCTGCTCCGCGTGTCCCCTCCCT TCCTCTGAGCCGGACTCGGTGCGTCTAAGATGAGGGAGTCAGGCCGTGGTGGTTTCTCCTTGAGACCGAG AGACTTTTCCGCGGAGCTGAGCTGGGGGCCCGGCAGTACTAGTATTAAGGAAGTAACCTTGTGCCTTGGA TACTCAAAACTCGCTCCTTTTCCTACCGAGTAGGGGGAGCAAAAATGTGCCTTGATATTTTATTTGGAGG ATTCCTGCTTCCTCTCGGGCCTCAGCTGGCCCCCGTGAGAAAAATGAAGGGTGCAGGCCCAGGGCAGGAG GAAGATACAGGAAGCTGAGATCCCGGCAGTGCCCTGAGCTGCCCCTCAGTCCCTGTCTTTAGAGGGGAGG GACTTAGGTGTTGGGGATTTGAGTCTGTGTCCTCACCCCCAGCTACAGGGAGGTGGAGGGCTCCTAATCC CTTGCTTTTTGCACCTCCACCTACATCCCCCCCCCCCACTCAGCTTACAACAGGCCAGGTTTCCTGGGTG AGTTCATGGAGAATGGGGGCACCACCCCCAGTCAGA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4194023), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 18272. Forward Primer - name:018272_F_IRAV24-27_L08_Cebpa, sequence:GCGCAAGAGCCGAGATAA; Reverse Primer - name:018272_R_SP6_IRAV24-27_L08_Cebpa, sequence:GTCTGACTGGGGGTGGTG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8619 same embryo
	EMAGE:8618 same embryo
	EMAGE:8623 same embryo
	EMAGE:8622 same embryo
	EMAGE:8620 same embryo
	EurExpress:euxassay_009855 same experiment
	MGI:4823796 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source