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EMAGE:8625

Mup4 major urinary protein 4 ( MGI:97236)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8625 EMAGE:8625 EMAGE:8625 EMAGE:8625 EMAGE:8625
"Pseudo-wholemount" of euxassay_009833. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009833_01 euxassay_009833_02 euxassay_009833_03 euxassay_009833_04
EMAGE:8625 EMAGE:8625 EMAGE:8625 EMAGE:8625 EMAGE:8625
euxassay_009833_05 euxassay_009833_06 euxassay_009833_07 euxassay_009833_08 euxassay_009833_09
EMAGE:8625 EMAGE:8625 EMAGE:8625 EMAGE:8625 EMAGE:8625
euxassay_009833_10 euxassay_009833_11 euxassay_009833_12 euxassay_009833_13 euxassay_009833_14
EMAGE:8625
euxassay_009833_15

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8625Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8625_wholemount_strong.wlz
8625_wholemount_moderate.wlz
8625_wholemount_weak.wlz
8625_wholemount_possible.wlz
8625_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8625_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
dermis
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10
tail
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30882
Entity Detected:Mup4, major urinary protein 4 ( MGI:97236)
Sequence:sense strand is shown

>T30882
GAAGGACGTGGTCCTGACAGACAGACAATTCTACTCCCTACCAAAATGAAGCTGCTGCTGTGTTTGGGAC
TGACCCTAGTCTGTATTCATGCAGAAGAAGCTACTTCCAAGGGACAGAACTTAAATGTAGAAAAGATTAA
TGGGGAATGGTTTTCTATTCTCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGAACATGGCAGCATGAGA
GTTTTTGTGGAGCACATCCATGTCTTGGAGAATTCCTTAGCTTTTAAATTCCATACTGTTATAGATGGAG
AGTGCTCTGAAATATTTTTGGTTGCTGACAAAACAGAGAAGGCTGGTGAATATTCTGTGATGTATGATGG
ATTCAATACATTTACTATACTTAAGACAGACTATGATAATTATATTATGTTTCATCTCATTAACGAAAAG
GATGGGAAAACCTTCCAGCTGATGGAGCTCTATGGCCGAAAAGCAGATTTGAATTCAGACATCAAGGAAA
AGTTTGTAAAACTTTGTGAGGAGCATGGAATCATTAAAGAAAATATCATTGACTTAACCAAAACCAATCG
CTGCCTCAAGGCCCGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCACCAGGACT
CCAGCATCCTCTCTTCCGATCGATACAGCATTGGGTGACAAGTTCTGTGATCTGCTTTCCATCCTGTCTC
ACAGAGAAGTGCAATCCTGGTCTCTCCAGCAT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4190774), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 35344. Forward Primer - name:035344_F_IRAV24-27_H10_Mup4, sequence:GAAGGACGTGGTCCTGACA; Reverse Primer - name:035344_R_SP6_IRAV24-27_H10_Mup4, sequence:CATGCTGGAGAGACCAGGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8627 same embryo
 EMAGE:8626 same embryo
 EMAGE:8624 same embryo
 EMAGE:8628 same embryo
 EMAGE:8629 same embryo
 EurExpress:euxassay_009833 same experiment
 MGI:4826512 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS