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EMAGE:8627

Bcar1 breast cancer anti-estrogen resistance 1 ( MGI:108091)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8627 EMAGE:8627 EMAGE:8627 EMAGE:8627 EMAGE:8627
"Pseudo-wholemount" of euxassay_009821. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009821_01 euxassay_009821_02 euxassay_009821_03 euxassay_009821_04
EMAGE:8627 EMAGE:8627 EMAGE:8627 EMAGE:8627 EMAGE:8627
euxassay_009821_05 euxassay_009821_06 euxassay_009821_07 euxassay_009821_08 euxassay_009821_09
EMAGE:8627 EMAGE:8627 EMAGE:8627 EMAGE:8627 EMAGE:8627
euxassay_009821_10 euxassay_009821_11 euxassay_009821_12 euxassay_009821_13 euxassay_009821_14
EMAGE:8627 EMAGE:8627 EMAGE:8627 EMAGE:8627 EMAGE:8627
euxassay_009821_15 euxassay_009821_16 euxassay_009821_17 euxassay_009821_18 euxassay_009821_19
EMAGE:8627 EMAGE:8627 EMAGE:8627 EMAGE:8627
euxassay_009821_20 euxassay_009821_21 euxassay_009821_22 euxassay_009821_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8627Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8627_wholemount_strong.wlz
8627_wholemount_moderate.wlz
8627_wholemount_weak.wlz
8627_wholemount_possible.wlz
8627_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8627_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
olfactory cortex marginal layer
moderate moderate
regionalmoderate expression: see section 08 09 10 11 14 15 16 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30617
Entity Detected:Bcar1, breast cancer anti-estrogen resistance 1 ( MGI:108091)
Sequence:sense strand is shown

>T30617
CCCAGCAGCTGAGGATGTATATGATGTGCCCCCCCCTGCTCCCGACCTCTATGATGTGCCCCCTGGTTTG
CGGCGGCCTGGCCCTGGAACACTGTATGACGTGCCTCGCGAGCGCGTGCTCCCTCCTGAGGTGGCTGATG
GGAGTGTAGTTGACGATGGTGTGTATGCTGTGCCCCCACCAGCCGAGCGAGAGGCCCCAACGGATGGCAA
GCGCCTGTCGGCCTCTAGCACGGGCAGCACGCGCAGCAGCCAATCGGCATCTTCCTTGGAGGTGGTGGTG
CCAGGCCGGGAGCCCCTGGAACTGGAGGTTGCTGTGGAGTCCCTGGCTCGGCTGCAGCAAGGCGTGAGCA
CCACCGTAGCCCACCTTCTGGACCTGGTGGGCAGTGCCAGTGGGCCTGGGGGCTGGCGTGGTACCTCTGA
GCCTCAGGAGCCTCCCGCACAGGACCTGAAAGCCGCAGTGGCGGCAGTTCACGGGGCAGTCCATGAGCTC
CTGGAGTTCGCCCGCGGAGCTGTGAGCAATGCCACCCACACTTCTGACCGCACCTTGCATGCCAAGCTTA
GCCGGCAACTACAGAAGATGGAGGATGTGTACCAGACACTGGTGGTCCACGGTCAGGTCCTTGACAGTGG
CCGAGGAAGTCCAGGATTCACTCCGGAAGACCTGGACCGCCTGGTGGCCTGCTCTCGGGCTGTGCCCGAG
GATGCCAAGCAGCTAGCTTCCTTTTTGCATGGCAATGCCTCGCTGCTTTTCAGACGGACCAAAGCCCCTG
GCCCAGGGCCTGAGGGAAGCAGCTCCCTGCACCCAAACCCCACTGATAAAGCCAGTAGCATCCAGTCACG
CCCTCTCCCCTCACCTCCAAAGTTCACCTCCCAGGACTCTCCAGACGGCCAGTATGAGAACAGTGAAGGG
GGTTGGATGGAGGACTATGACTACGTCCATCTGCAGGGGAAGGAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6401186), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59736. Forward Primer - name:059736_F_IRAV115_b11_Bcar1, sequence:CCCAGCAGCTGAGGATGT; Reverse Primer - name:059736_R_SP6_IRAV115_b11_Bcar1, sequence:CCTCCTTCCCCTGCAGAT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8626 same embryo
 EMAGE:8624 same embryo
 EMAGE:8625 same embryo
 EMAGE:8628 same embryo
 EMAGE:8629 same embryo
 EurExpress:euxassay_009821 same experiment
 MGI:4823438 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS