EMAGE mouse gene expression spatial database

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EMAGE:863

Pitx1 paired-like homeodomain transcription factor 1 ( MGI:107374)
TS13 (8.5 dpc)
in situ hybridisation

Data Images


Figure 6B of Lanctot et al., 1997 [PMID:9226452] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.	Figure 6A of Lanctot et al., 1997 [PMID:9226452] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity:
Find spatially similar expression patterns:

Notes:

Image A is a brightfield image of the section shown in Image B. Image annotations: am - amnion; hg - hindgut; nt - neural tube/plate; sm - somatic layer of lateral plate mesoderm; sp - splanchnic layer of lateral plate mesoderm; va - vitelline artery.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

3D mapping

3D context movie

View mapped 3D expression image

Download individual expression domains:

863_voxel_strong_3D_1.wlz

863_voxel_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:863_all_domains.zip

Find spatially similar expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hindgut diverticulum endoderm

possible

somatopleure

detected

Expression extends up to the posterior intestinal portal

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:2150936
Entity Detected:	Pitx1, paired-like homeodomain transcription factor 1 ( MGI:107374)
Sequence:	sense strand is shown >MGI:2150936 GGGAGCCGCTGGAGAACTCCGCCAGCGAATCGTCCGACGCTGATCTGCCAGACAAGGAGCGCGGTGGGGA AGCCAAGGGGCCAGAGGATGGTGGCGCGGGCAGTGCTGGCTGCGGCGGCGGTGCAGAGGACCCAGCTAAG AAGAAGAAACAGCGGCGGCAACGCACTCACTTCACAAGCCAGCAGTTGCAAGAGCTGGAGGCCACGTTCC AAAGGAACCGCTACCCCGACATGAGCATGAGAGAGGAGATCGCGGTGTGGACCAACCTCACTGAACCGCG AGTGCGGGTCTGGTTCAAGAACCGGCGAGCCAAATGGCGCAAGCGGGAGCGGAACCAGCAGTTGGACCTG TGCAAGGGCGGCTATGTGCCGCAGTTCAGCGGCCTGGTGCAGCCCTACGAGGACGTGTACGCTGCCGGCT ACTCCTACAACAACTGGGCGGCCAAGAGCCTGGCCCCGGCGCCGCTGTCTACCAAGAGCTTTACCTTCTT CAACTCCATGAGCCCGCTCTCCTCTCAATCCATGTTCTCGGCCCCCAGCTCCATCTCTTCCATGACCATG CCGTCCAGCATGGG
	nt 604 - nt 1177 of U71206.1
Notes:	The probe used in this study by Lanctot et al., 1997 [PMID:9226452] is described as follows: "Two cDNA fragments were transcribed into cRNA probes: a 573 nt fragment (from nt 604-1177) encompassing the homeodomain and a 310 nt fragment encoding the last 14 amino acids and 268 bp of 3' untranslated region. Most figures presented here were obtained using the longest cRNA probe because it gave a stronger signal; however, labelling was identical with the 3' probe".
Chemistry:	RNA
Strand:	antisense
Label:	S35

Specimen

Organism:	mouse
Strain:	CBA x C57BL/6
Age:	8.5 dpc
Theiler Stage:	TS13
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	4% paraformaldehyde
Embedding:	paraffin
Staining procedure:	autoradiography

General Information

Authors:	Lanctot et al., 1997 [PMID:9226452] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ PMID:9226452] Lanctot C, Lamolet B, Drouin J 1997 The bicoid-related homeoprotein Ptx1 defines the most anterior domain of the embryo and differentiates posterior from anterior lateral mesoderm. Development (124):2807-17
Links:	MGI:2151020 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source