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EMAGE:8638

Atp5o ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit ( MGI:106341)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8638 EMAGE:8638 EMAGE:8638 EMAGE:8638 EMAGE:8638
"Pseudo-wholemount" of euxassay_009851. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009851_01 euxassay_009851_02 euxassay_009851_03 euxassay_009851_04
EMAGE:8638 EMAGE:8638 EMAGE:8638 EMAGE:8638 EMAGE:8638
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EMAGE:8638 EMAGE:8638 EMAGE:8638 EMAGE:8638 EMAGE:8638
euxassay_009851_10 euxassay_009851_11 euxassay_009851_12 euxassay_009851_13 euxassay_009851_14
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euxassay_009851_15 euxassay_009851_16 euxassay_009851_17 euxassay_009851_18 euxassay_009851_19
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View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8638Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8638_wholemount_strong.wlz
8638_wholemount_moderate.wlz
8638_wholemount_weak.wlz
8638_wholemount_possible.wlz
8638_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8638_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30896
Entity Detected:Atp5o, ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit ( MGI:106341)
Sequence:sense strand is shown

>T30896
ACTGTCCCGACAGGTTCGGAGCTTCAGTACATCTGTGGTCAGGCCCTTTGCCAAGCTTGTAAGGCCCCCT
GTTCAGGTCTACGGCATCGAAGGCCGCTATGCAACCGCCCTGTACTCTGCTGCATCTAAGGAGAAGAAGC
TGGACCAGGTGGAGAAGGAGCTGCTGCGCGTAGGGCAACTCCTGAAGGACCCCAAAGTGTCTCTGGCTGT
TCTGAATCCCTACATCAAGCGCACCGTCAAAGTGAAAAGCCTAAATGACATCACGAAAAGGGAAAAGTTC
TCCCCGCTGACGGCCAACCTCATGAATTTACTTGCTGAAAATGGTCGCCTAGGCAACACCCAGGGTATCA
TCTCTGCCTTTTCCACCATCATGAGTGTCCACCGCGGAGAAGTGCCGTGCACAGTGACCACAGCATCTCC
TCTAGATGACGCTGTTCTCTCTGAGTTAAAGACGGTGCTGAAGAGCTTCCTGAGTCCAAACCAAATACTG
AAACTGGAGATCAAGACTGACCCGTCAATCATGGGCGGGATGATTGTCCGAATTGGGGAAAAGTACGTTG
ATATGTCTGCAAAGAGCAAGATTCAGAAGCTCAGCAAGGCCAT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4023318), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 35449. Forward Primer - name:035449_F_IRAV24-27_J11_Atp5o, sequence:ACTGTCCCGACAGGTTCG; Reverse Primer - name:035449_R_SP6_IRAV24-27_J11_Atp5o, sequence:CATGGCCTTGCTGAGCTT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8641 same embryo
 EMAGE:8639 same embryo
 EMAGE:8637 same embryo
 EMAGE:8636 same embryo
 EMAGE:8640 same embryo
 EurExpress:euxassay_009851 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS