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EMAGE:8643

Ddx60 DEAD (Asp-Glu-Ala-Asp) box polypeptide 60 ( MGI:2384570)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8643 EMAGE:8643
euxassay_009852_01 euxassay_009852_02

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8643Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8643_wholemount_possible.wlz
8643_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8643_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30912
Entity Detected:Ddx60, DEAD (Asp-Glu-Ala-Asp) box polypeptide 60 ( MGI:2384570)
Sequence:sense strand is shown

>T30912
GGCAAGACCTGTTGGGTGATGTCTACTTCTTTGATATTCCACTGCCCAAAATAGGAAAACTCATAAAATC
CAAAGTTCCTGAACTTAGAGGACAGTTTCCCCTCAGTATTTCACTCATCCTAAGACTCATGTTGCTGGCT
TCCAAGGCGGATGACCTAGAGGATGGCAAGGCAAAGGCACTGTCAGTGCTGAAGCATTCATTGTTATCCT
TCAAACAACCCCGAGCCATAGACATGTTAAAGCTTTACTTCTTATATTCTTTGCAGTTCCTGGTGAAAGA
GGGACATATAGATCAAGAAGGTAATCCTACAGGGTTTGCTGGCCTTGTCTCCCATTTACATTACCACGAA
CCTTCTAATCTCGTGTTTGTCAGTTTTCTTGTAAAAGGCCTTTTCCATAAACTTTGTCAGCCAACAAGGG
AAGGCCCAAAACGTTTTTCTGAAGATGTAATGGAAAAGCTGGTTTTGGTATTGGCAAATCTTTTTGGAAG
AAGATATATCCCAGCCAAGTTCTATGAGGTTAACTCCAAGTTTTATCAATCAAAGGTGTTCCTTGATGAC
CTTCCCGAGGACTTCAGTCACGCCCTGCTTGAGTACAATACGCAAGTGACAGAAGACTTCGCTTCCTTTC
TGCTAATGGTATCCCGATTGGCTGATATGAAACAGGAGTATCAACTCTCCTTGTCAAAAATCGAGTTCAC
AGGTAAAGAATGTGAAGATTCGCCACTTGTGTCTCACTTGATGAACTGCAAGGAAGGAAGGGTGGCGGTT
TCACCCTTTGTTTGTCTGTCTGGAAACTTTGATGTGGATTTGCTTAGTCCAGGAGTTCCAAATCATGTCA
TTCTACACACACTTGGTATCAATCACACACAAGCCCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4037887), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 19592. Forward Primer - name:019592_F_IRAV24-27_N07_BC013672, sequence:GGCAAGACCTGTTGGGTG; Reverse Primer - name:019592_R_SP6_IRAV24-27_N07_BC013672, sequence:CGGGGCTTGTGTGTGATT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8647 same embryo
 EMAGE:8642 same embryo
 EMAGE:8646 same embryo
 EMAGE:8645 same embryo
 EMAGE:8644 same embryo
 EurExpress:euxassay_009852 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS