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EMAGE:865

Pdgfra platelet derived growth factor receptor, alpha polypeptide ( MGI:97530)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:865 EMAGE:865
Figure 5J of Orr-Urtreger & Lonai, 1992 [PMID:1451656] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 5I of Orr-Urtreger & Lonai, 1992 [PMID:1451656] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image I is a brightfield image of a consecutive section to that shown in Image J. Image annotations: my - myelocoel; ov - otic vesicle; ph - pharynx; ma - mandibular arch.
Expression Pattern Description
Spatial Annotation:
EMAGE:865EMAGE:865Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
865_voxel_strong_3D_1.wlz
865_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:865_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
branchial arch
detected detected
regionalExpression in the underlying branchial arch mesenchyme of the pharynx.
otocyst
detected detected
regionalExpression in the mesenchyme surrounding the otic vesicle.
head mesenchyme
detected detected
regionalExpression in the mesenchyme surrounding the otic vesicle
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1929663
Entity Detected:Pdgfra, platelet derived growth factor receptor, alpha polypeptide ( MGI:97530)
Notes:The Pdgfra probe used in this study by Orr-Urtreger & Lonai, 1992 [PMID:1451656] is described as "a 359 bp long cDNA fragment isolated from the interkinase region of the murine Pdgfra cDNA as described previously (Do et al., 1992 [PMID:1321404] )." The sites of the ends points are not mentioned.
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Strain:C57BL/6J
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:cryosection
Staining procedure:autoradiography
General Information
Authors:Orr-Urtreger & Lonai, 1992 [PMID:1451656] , Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:1451656] Orr-Urtreger A, Lonai P 1992 Platelet-derived growth factor-A and its receptor are expressed in separate, but adjacent cell layers of the mouse embryo. Development (115):1045-58
 [ PMID:1321404] Do MS, Fitzer-Attas C, Gubbay J, Greenfeld L, Feldman M, Eisenbach L 1992 Mouse platelet-derived growth factor alpha receptor: sequence, tissue-specific expression and correlation with metastatic phenotype. Oncogene (7):1567-75
Links:MGI:1929710 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI