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EMAGE:8655

Dgkg diacylglycerol kinase, gamma ( MGI:105060)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8655 EMAGE:8655 EMAGE:8655 EMAGE:8655 EMAGE:8655
"Pseudo-wholemount" of euxassay_009859. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009859_01 euxassay_009859_02 euxassay_009859_03 euxassay_009859_04
EMAGE:8655 EMAGE:8655 EMAGE:8655 EMAGE:8655 EMAGE:8655
euxassay_009859_05 euxassay_009859_06 euxassay_009859_07 euxassay_009859_08 euxassay_009859_09
EMAGE:8655 EMAGE:8655 EMAGE:8655 EMAGE:8655 EMAGE:8655
euxassay_009859_10 euxassay_009859_11 euxassay_009859_12 euxassay_009859_13 euxassay_009859_14
EMAGE:8655 EMAGE:8655 EMAGE:8655 EMAGE:8655 EMAGE:8655
euxassay_009859_15 euxassay_009859_16 euxassay_009859_17 euxassay_009859_18 euxassay_009859_19
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euxassay_009859_20 euxassay_009859_21 euxassay_009859_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8655Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8655_wholemount_strong.wlz
8655_wholemount_moderate.wlz
8655_wholemount_weak.wlz
8655_wholemount_possible.wlz
8655_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8655_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
vagus x ganglion
strong strong
spottedstrong expression: see section 05 13 14 moderate expression: see section 04
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30923
Entity Detected:Dgkg, diacylglycerol kinase, gamma ( MGI:105060)
Sequence:sense strand is shown

>T30923
GCTGGTCCTCCTGGGAATGGATGACTCTGGCTCCAAGGGCGATGGGAGGCATGCCTGGACCCTGAAGCAC
TTCAAGAAGCCCACTTACTGCAACTTCTGCCGCGCCATGCTGATGGGTGTTGGGAAGCAAGGCCTGTGCT
GCATCTACTGTAAATATACAGTCCACCAACGCTGTGTATCCAAAACCATTCATGGATGTGTGAAAACAAA
CTCCAAAGCCAAAAGGAGTGGCGAGGTGATGCAGCATGCGTGGGTGGAAGGGAACTCCTCCGTCAAGTGT
GACCGGTGCCACAAAAGTATCAAGTGCTACCAGAGTGTCACCGCGCGGCACTGCGTGTGGTGCCGGATGA
CGTTTCACCGCAAATGTGAATTATCGACAGTGTGCGACGGTGGGGAACTCAAAGACCACATTTTGCTGCC
TACCTCCATATGCCCCGTCTCCGGAGACCGGCAAGGAGGGAAGTCCGATGGCAGTGTGGCCGCCAAGGGT
GAACTTGTAACTCAGTATAAGATCATCCCTAGCCCGGGGACGCATCCCCTGCTGGTCCTGGTGAACCCCA
AGAGTGGAGGAAGACAAGGAGAAAGAATTCTTCGGAAATTTCACTACCTGCTCAACCCCGAACAAGTTTT
CAACCTGGACAATGGGGGACCTACACCAGGATTGAACTTCTTTCATGATACCCCAGACTTCCGTGTTCTT
GCCTGTGGCGGAGATGGGACCGTTGGCTGGATTTTGGATTGCATTGATAAGGCTAACTTCACAAAGCACC
CACCGGTGGCTGTCCTGCCTCTTGGAACAGGGAATGACCTCGCACGATGCCTCCGCTGGGGAGGAGGTTA
CGAAGGGGGCAGCTTGACCAAAATCCTGAAGGAAATTGAACAGAGCCCCTTGGTGATGCTGGA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4221290), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 18611. Forward Primer - name:018611_F_IRAV28-31_B08_Dgkg, sequence:GCTGGTCCTCCTGGGAAT; Reverse Primer - name:018611_R_SP6_IRAV28-31_B08_Dgkg, sequence:GTCCAGCATCACCAAGGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8656 same embryo
 EMAGE:8658 same embryo
 EMAGE:8654 same embryo
 EMAGE:8657 same embryo
 EurExpress:euxassay_009859 same experiment
 MGI:4824290 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS