EMAGE mouse gene expression spatial database

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EMAGE:8656

Cbr2 carbonyl reductase 2 ( MGI:107200)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009854. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009854_01	euxassay_009854_02	euxassay_009854_03	euxassay_009854_04

euxassay_009854_05	euxassay_009854_06	euxassay_009854_07	euxassay_009854_08	euxassay_009854_09

euxassay_009854_10	euxassay_009854_11	euxassay_009854_12	euxassay_009854_13	euxassay_009854_14

euxassay_009854_15	euxassay_009854_16	euxassay_009854_17	euxassay_009854_18	euxassay_009854_19

euxassay_009854_20	euxassay_009854_21	euxassay_009854_22	euxassay_009854_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8656_wholemount_strong.wlz

8656_wholemount_moderate.wlz

8656_wholemount_weak.wlz

8656_wholemount_possible.wlz

8656_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8656_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 06 07 08 09 10 12 13 14 15 16

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 08 09 13 not examined expression: see section 15

esophagus epithelium

moderate

regional

moderate expression: see section 11 12 weak expression: see section 09 10

tongue epithelium

strong

regional

strong expression: see section 06 07 08 09 10 11 12

not examined

regional

not examined expression: see section 12

palatal shelf epithelium

strong

regional

strong expression: see section 08 09 10 11 12

trachea epithelium

weak

regional

weak expression: see section 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30922
Entity Detected:	Cbr2, carbonyl reductase 2 ( MGI:107200)
Sequence:	sense strand is shown >T30922 AGGGAAAGGGATTGGACGGGACACTGTGAAGGCCCTGCACGCCTCAGGAGCCAAAGTGGTGGCGGTGACT CGGACCAACTCAGACCTGGTCAGCCTTGCCAAAGAGTGTCCCGGAATAGAGCCCGTGTGTGTGGACCTGG GTGACTGGGATGCCACAGAGAAGGCGCTGGGCGGTATCGGCCCTGTGGACTTACTGGTGAACAATGCAGC GCTGGTTATAATGCAGCCCTTCCTGGAAGTTACCAAGGAAGCCTTTGACAGGTCCTTCAGTGTAAATCTT CGCTCTGTGTTTCAGGTATCCCAGATGGTAGCCAGGGACATGATTAACCGTGGCGTGCCAGGGTCCATTG TCAATGTCTCCAGCATGGTGGCCCATGTCACCTTTCCTAACTTGATCACCTACAGCTCCACCAAGGGAGC GATGACTATGCTGACCAAAGCCATGGCCATGGAACTAGGGCCACACAAGATCCGGGTAAACTCCGTAAAC CCCACTGTGGTGCTGACTGACATGGGCAAGAAAGTCTCTGCAGACCCTGAGTTTGCCAGGAAGCTCAAGG AGCGCCACCCACTGAGGAAGTTCGCAGAGGTTGAAGACGTCGTCAACAGCATCCTCTTCCTGCTCAGCGA CAGAAGTGCCTCTACCAGTGGCGGTGGCATCCTGGTGGATGCTGGCTACCTGGCCTCCTAGTCTGCTCAG TTGCCGCGGACATCTGAGTGGCCTTCTTAGCCCCACCCTCAGCCAAAGCATTTACTGATCTCGTGACTCC GCCCTCATGCTACAGCCACGCCCACCACGCAGCTCACAGTTCCACCCCCATGTTACTGTCGATCCCACAA CCACTCCAGGCGCAGACCTTGTTCTCTTTGTCCACTTTGTTGGGCTCATTTGCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4219349), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 17770. Forward Primer - name:017770_F_IRAV28-31_B02_Cbr2, sequence:AGGGAAAGGGATTGGACG; Reverse Primer - name:017770_R_SP6_IRAV28-31_B02_Cbr2, sequence:AGGCAAATGAGCCCAACA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8655 same embryo
	EMAGE:8658 same embryo
	EMAGE:8654 same embryo
	EMAGE:8657 same embryo
	EurExpress:euxassay_009854 same experiment
	MGI:4823656 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source