EMAGE mouse gene expression spatial database

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EMAGE:8657

Mall mal, T-cell differentiation protein-like ( MGI:2385152)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009865. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009865_01	euxassay_009865_02	euxassay_009865_03	euxassay_009865_04

euxassay_009865_05	euxassay_009865_06	euxassay_009865_07	euxassay_009865_08	euxassay_009865_09

euxassay_009865_10	euxassay_009865_11	euxassay_009865_12	euxassay_009865_13	euxassay_009865_14

euxassay_009865_15	euxassay_009865_16	euxassay_009865_17	euxassay_009865_18	euxassay_009865_19

euxassay_009865_20	euxassay_009865_21	euxassay_009865_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8657_wholemount_strong.wlz

8657_wholemount_moderate.wlz

8657_wholemount_weak.wlz

8657_wholemount_possible.wlz

8657_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8657_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

naris

weak

regional

weak expression: see section 07 08 10 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30926
Entity Detected:	Mall, mal, T-cell differentiation protein-like ( MGI:2385152)
Sequence:	sense strand is shown >T30926 CCATCCCCTTTGCCTTCTTCCTACCGGAGCTGGTGTTCGGGTTCTGGGTGTGGACTCTGGTGGCTGCTAC ACACGTCGCATACCCCTTGCTGCAGGGATGGGTGCTGTATGTCTCCCTCACCTCCTTTCTCATCTCCTTG ATGTTCCTGATGTCCTACTTGTTTGGGTTTTACAAACGATTTGAGTCCTGGAGAGTCCTGGACAGCCTGT ACCATGGCACCACAGGCATTTTGTACATGAGTGCCTCTGTCCTGCAAGCATATGCCACCATCATTTCTGA GGGCCACAACCTGAGCCACTACTACATCAATGTTGCAGCCTCGTTCTTCGCCTTCCTCACTACACTGCTC TATATCCTGCACGCTTTCAGCATCTATTACCACTGAAGTGCAGGGAGAACCAAATCCGGCGAGACCTGAG AGGCCGCGTGATTATCCCTCAAAGCAGGTCTTGTCATTTGCCAACTGGGTGATAAACCCACCAAGCCACC CGTGGGGCCAACAGACCATCTTTCTGGAACGATGAGGAGACTCATTTTGGACCTTTATACTCTTTGTGGT GACTATGAGAAAACTATCCCCCCACCAAAGATGACTGCCCCTCCTTCTCCCCTGAAGATCCCTCCCACCC TGGCTGCCCTGAAGATGACGGGCTGCCTTTTGGAGTTTCCCAACCGTGAACTCAGCTGAGCTCGGCTTTT CTAGAAAACTAACCTTGAACCTCTCAGGCACCGTTCAGAATGCCACCCCTGCTGTTGCCCCCATCCAGGA TCTCTTTGTTAATGGTGCTCTGTCTGATACATTTAGGTCTTAGTCAATCTTTTCTCAAACAGGCAAGTCT ACACAAAGGCTTGCCGGTCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4205488), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 19156. Forward Primer - name:019156_F_IRAV28-31_C09_BC012256, sequence:CCATCCCCTTTGCCTTCT; Reverse Primer - name:019156_R_SP6_IRAV28-31_C09_BC012256, sequence:TAGACCGGCAAGCCTTTG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8656 same embryo
	EMAGE:8655 same embryo
	EMAGE:8658 same embryo
	EMAGE:8654 same embryo
	EurExpress:euxassay_009865 same experiment
	MGI:4826068 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source