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EMAGE:866

Pdgfra platelet derived growth factor receptor, alpha polypeptide ( MGI:97530)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:866 EMAGE:866
Figure 3B of Orr-Urtreger & Lonai, 1992 [PMID:1451656] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 3A of Orr-Urtreger & Lonai, 1992 [PMID:1451656] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: no stars
Notes:
Image A is a brightfield image of the section shown in Image B. Image annotations: sc - spinal cord; AER - apical ectodermal ridge.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
forelimb bud
detected detected
Diffuse pattern of expression in the limb bud mesenchyme.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1929663
Entity Detected:Pdgfra, platelet derived growth factor receptor, alpha polypeptide ( MGI:97530)
Notes:The Pdgfra probe used in this study by Orr-Urtreger & Lonai, 1992 [PMID:1451656] is described as "a 359 bp long cDNA fragment isolated from the interkinase region of the murine Pdgfra cDNA as described previously (Do et al., 1992 [PMID:1321404] )." The sites of the ends points are not mentioned.
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Strain:C57BL/6J
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:cryosection
Staining procedure:autoradiography
General Information
Authors:Orr-Urtreger & Lonai, 1992 [PMID:1451656] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:1451656] Orr-Urtreger A, Lonai P 1992 Platelet-derived growth factor-A and its receptor are expressed in separate, but adjacent cell layers of the mouse embryo. Development (115):1045-58
 [ PMID:1321404] Do MS, Fitzer-Attas C, Gubbay J, Greenfeld L, Feldman M, Eisenbach L 1992 Mouse platelet-derived growth factor alpha receptor: sequence, tissue-specific expression and correlation with metastatic phenotype. Oncogene (7):1567-75
Links:MGI:1929710 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI