EMAGE mouse gene expression spatial database

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EMAGE:8668

Zfpm2 zinc finger protein, multitype 2 ( MGI:1334444)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009941. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009941_01	euxassay_009941_02	euxassay_009941_03	euxassay_009941_04

euxassay_009941_05	euxassay_009941_06	euxassay_009941_07	euxassay_009941_08	euxassay_009941_09

euxassay_009941_10	euxassay_009941_11	euxassay_009941_13	euxassay_009941_14	euxassay_009941_15

euxassay_009941_16	euxassay_009941_17	euxassay_009941_18	euxassay_009941_19	euxassay_009941_20

euxassay_009941_21	euxassay_009941_22	euxassay_009941_23	euxassay_009941_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8668_wholemount_strong.wlz

8668_wholemount_moderate.wlz

8668_wholemount_weak.wlz

8668_wholemount_possible.wlz

8668_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8668_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 07 weak expression: see section 08 09 10 11 15 16 17 18

cerebral cortex mantle layer

moderate

regional

moderate expression: see section 01 02 09 10 11 12 13 14 17 18 19 20 21 weak expression: see section 03 04 05 06 07 08 15 16 22 23 24

medulla oblongata basal plate mantle layer

moderate

spotted

moderate expression: see section 11 15 weak expression: see section 09 10 12 14

pons mantle layer

moderate

regional

moderate expression: see section 09 10 11 14 15 16 17

midbrain mantle layer

moderate

regional

moderate expression: see section 05 06 07 08 09 15 16 17 18 19 weak expression: see section 10 11 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30616
Entity Detected:	Zfpm2, zinc finger protein, multitype 2 ( MGI:1334444)
Sequence:	sense strand is shown >T30616 CGGACGACAGCATCTCCTAAGAGGCTACTAGACTACCACGAGTGCACAGTGTGCAAGATCAGCTTCAATA AGGTCGAGAACTATCTGGCGCACAAGCAGAATTTCTGCCCTGTCACTGCACATCAGCGTAATGACCTGGG TCAGCTTGATGGTAAAGTGTTTCCGAATCCAGAAAGTGAACGGAGCAGCCCTGAAGTCAGCTTTGAAAGA AACATGATCAAATGTGAGAAGAATGGGAATCCAAAGCAACCATCTCCCAACGGAAATTTGTTTTCATCCC ACTTGGCAACCTTGCAAGGCCTGAAAGTCTTCAGCGAAGCAGCTCAGCTCATTGCTACAAAAGAAGAAAA CAAACATTTGTTTCTTCCCCAATGCCTTTACCCTGGAGCAATAAAGAAGACAAAAGGAGCTGACCAACTT TCTCCATACTATGGGATAAAGCCAAGCGATTATATCGCCAGTTCTCTTGTCATCCACAACACTGACGTCG AACAAAGCACAAATACAGAAAATGAATCTCCTAAAGGCCAGGCCTCCTCAAATGGGTGTGCCGTACCCAA GAAAGATTCTCTGCCATTGTTGCCCAAAAACAGAGGCATGGTCATAGTGAATGGTGGACTCAAGCAAGAT GAAAGACCTACGGCCAACCCACAGCAAGAGAACATTTCCCAGAATACCCAGCACGAAGATGGCCACAAAT CCCCTTCGTGGATCTCTGAGAACCCATTAGCTGCAAATGAGAATGTCTCCCCAGGAATTCCCTGCGCAGA AGAACAGTTGTCTAGCATAGCAAAAGGTGTGAATGGCGCCAGCCAGGCTCCCAGCAGTGGAAA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6408975), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59715. Forward Primer - name:059715_F_IRAV115_b06_Zfpm2, sequence:CGGACGACAGCATCTCCT; Reverse Primer - name:059715_R_SP6_IRAV115_b06_Zfpm2, sequence:TTTTCCACTGCTGGGAGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8670 same embryo
	EMAGE:8667 same embryo
	EMAGE:8669 same embryo
	EMAGE:8666 same embryo
	EMAGE:8665 same embryo
	EurExpress:euxassay_009941 same experiment
	MGI:4829347 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source