EMAGE mouse gene expression spatial database

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EMAGE:8680

Pdc phosducin ( MGI:98090)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009925. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009925_01	euxassay_009925_02	euxassay_009925_03	euxassay_009925_04

euxassay_009925_05	euxassay_009925_06	euxassay_009925_07	euxassay_009925_08	euxassay_009925_09

euxassay_009925_10	euxassay_009925_11	euxassay_009925_12	euxassay_009925_13	euxassay_009925_14

euxassay_009925_15	euxassay_009925_16	euxassay_009925_17	euxassay_009925_18	euxassay_009925_19

euxassay_009925_20	euxassay_009925_21	euxassay_009925_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8680_wholemount_strong.wlz

8680_wholemount_moderate.wlz

8680_wholemount_weak.wlz

8680_wholemount_possible.wlz

8680_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8680_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

humerus

moderate

regional

moderate expression: see section 01 02 03 weak expression: see section 20 21 22

foot

moderate

regional

moderate expression: see section 03 weak expression: see section 22

fibula

weak

regional

weak expression: see section 22

tibia

moderate

regional

moderate expression: see section 01 weak expression: see section 22

femur

moderate

regional

moderate expression: see section 02 03 04 05 06 07 weak expression: see section 18 19 20 21 22

brain

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

spinal cord

weak

regional

weak expression: see section 08 09 10 11 12 13 14

spinal nerve plexus

moderate

regional

moderate expression: see section 16

brachial plexus

moderate

regional

moderate expression: see section 06

axial skeleton

moderate

regional

moderate expression: see section 08 09 12 13 14

basioccipital bone

moderate

regional

moderate expression: see section 02 03 04 05 17 18 19 20 21

scapula

moderate

regional

moderate expression: see section 01 02 03 weak expression: see section 20

pelvic girdle skeleton

moderate

regional

moderate expression: see section 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30959
Entity Detected:	Pdc, phosducin ( MGI:98090)
Sequence:	sense strand is shown >T30959 CCACCCAGCAAGAAGGAAATCCTCAGACAAATGTCCTCTCCTCAGAGCAGAGATGACTCAAAAGAAAGAA TGAGCAGAAAGATGAGCATTCAAGAATATGAACTCATTCATCAAGACAAAGAAGATGAGAGTTGCCTTCG CAAATACCGGAGGCAGTGCATGCAGGATATGCATCAGAAGTTGAGTTTTGGGCCTAGGTATGGGTTTGTG TATGAGCTGGAAACAGGGGAGCAATTCCTGGAAACCATCGAGAAGGAGCAGAAGGTCACCACAATTGTGG TTAACATTTACGAGGATGGTGTCAGGGGCTGCGATGCACTCAACAGCAGCTTAGCGTGCCTTGCAGTAGA GTACCCAATGGTCAAGTTCTGTAAAATAAAAGCTTCTAATACCGGAGCGGGGGACCGCTTTTCCACAGAC GTACTCCCTACGTTGCTGGTGTACAAAGGTGGGGAACTCATAAGCAATTTTATTAGTGTGGCTGAACAAT TTGCTGAAGAATTTTTTGCTGTGGATGTGGAGTCTTTCCTAAATGAATATGGATTACTACCAGAAAGAGA GATACATGACCTAGAGCAGACCAACATGGAAGACGAGGACATTGAATAAGCCTTCACCATGTCAGTATCT CATGTCTATCCTTTACACAGTGAACACTGATTTTTTTTTTTGGTAGTATCTATATTCCTTTAGCAAACAC TAAATATGGGCCTGGCTATTTAAACTCGGGGGGGGGGACACACTAAAATTATGTACATAGCACTTTTTAG TACTAATTACTCAAATTGAGATAATATTTTACTGCAAGGATATTGTGGTTTTTAGCAAATAACTAGTAAG CAGAGGAGGTGGGTAATGTCAATGTTTAGATCATTGTAAAAATCCCTTTCAAGTTCCGTGTTAGATTTTG CTCCTCATTTTTCCCTTGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4500900), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 19945. Forward Primer - name:019945_F_IRAV28-31_L16_Pdc, sequence:CCACCCAGCAAGAAGGAA; Reverse Primer - name:019945_R_SP6_IRAV28-31_L16_Pdc, sequence:GCCAAGGGAAAAATGAGGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8678 same embryo
	EMAGE:8681 same embryo
	EMAGE:8679 same embryo
	EMAGE:8682 same embryo
	EMAGE:8677 same embryo
	EurExpress:euxassay_009925 same experiment
	MGI:4827110 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source