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EMAGE:869

Jag1 jagged 1 ( MGI:1095416)
TS11 (7.5 dpc)
in situ hybridisation

Data Images
EMAGE:869
Fig 4D Przemeck et al, 2003 [PMID:12441287] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: the red circle indicates the approximate position of the node; the asterisk indicates the position of the allantois.
Expression Pattern Description
Spatial Annotation:
EMAGE:869Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
869_wholemount_strong_3D_1.wlz
869_wholemount_moderate_3D_1.wlz
869_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:869_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo mesoderm
detected detected
regionalExpression in an anterior domain
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1095488
Entity Detected:Jag1, jagged 1 ( MGI:1095416)
Notes:The Jag1 (Ser-1) probe used in this study by Przemeck et al, 2003 [PMID:12441287] is indicated as that used by Mitsiadis et al, [PMID:9108364] ie. "an 1,800 bp fragment of Ser-1 mouse cDNA, derived from the region encoding the extracellular domain of the Ser-1, was used". Mitsiadis et al also state "The nucleic acid sequence has been deposited with Genbank (X91012)" however X91012 corresponds to alpha 3 type IX collagen and not Jag1/Ser1.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:7.5 dpc
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Przemeck et al, 2003 [PMID:12441287] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.00176] [ PMID:12441287] Przemeck GK, Heinzmann U, Beckers J, Hrabe de Angelis M 2003 Node and midline defects are associated with left-right development in Delta1 mutant embryos. Development (130):3-13
 [ PMID:9108364] Mitsiadis TA, Henrique D, Thesleff I, Lendahl U 1997 Mouse Serrate-1 (Jagged-1): expression in the developing tooth is regulated by epithelial-mesenchymal interactions and fibroblast growth factor-4. Development (124):1473-83
Links:MGI:2449026 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI