EMAGE mouse gene expression spatial database

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EMAGE:8703

Sgpl1 sphingosine phosphate lyase 1 ( MGI:1261415)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009987. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009987_01	euxassay_009987_02	euxassay_009987_03	euxassay_009987_04

euxassay_009987_05	euxassay_009987_06	euxassay_009987_07	euxassay_009987_08	euxassay_009987_09

euxassay_009987_10	euxassay_009987_11	euxassay_009987_12	euxassay_009987_13	euxassay_009987_14

euxassay_009987_15	euxassay_009987_16	euxassay_009987_18	euxassay_009987_19	euxassay_009987_20

euxassay_009987_21	euxassay_009987_22	euxassay_009987_23	euxassay_009987_24	euxassay_009987_25

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8703_wholemount_strong.wlz

8703_wholemount_moderate.wlz

8703_wholemount_weak.wlz

8703_wholemount_possible.wlz

8703_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8703_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

thymus primordium

strong

regional

strong expression: see section 12 13 14 15 16

cerebral cortex mantle layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18 19 moderate expression: see section 10

urinary system

moderate

regional

moderate expression: see section 05 20 21

metanephros

moderate

regional

moderate expression: see section 04

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31150
Entity Detected:	Sgpl1, sphingosine phosphate lyase 1 ( MGI:1261415)
Sequence:	sense strand is shown >T31150 TACCGGGACTTGGCGTTAGAGAAGGGGATCAAAACTCCAGAAATTGTGGCTCCCGAGAGTGCCCATGCTG CATTCGACAAAGCAGCTCATTATTTTGGGATGAAGATTGTCCGAGTTGCACTGAAAAAGAACATGGAGGT GGATGTGCAGGCAATGAAGAGAGCCATCTCCAGGAACACAGCTATGCTGGTCTGTTCTACCCCACAGTTT CCTCATGGTGTGATGGATCCTGTCCCCGAAGTGGCCAAGTTGGCTGTCAGATATAAAATCCCACTCCATG TGGATGCTTGTCTGGGGGGCTTCCTCATTGTCTTCATGGAGAAAGCAGGGTACCCACTGGAGAAACCATT TGATTTCCGGGTGAAAGGTGTGACCAGCATTTCAGCAGATACTCATAAGTATGGCTATGCTCCTAAAGGT TCATCAGTGGTGATGTACTCTAACGAGAAGTACAGGACGTACCAGTTCTTTGTTGGTGCAGACTGGCAAG GTGGTGTCTACGCATCTCCAAGCATAGCTGGCTCACGGCCTGGTGGCATCATTGCAGCCTGTTGGGCCGC CTTGATGCACTTCGGTGAGAACGGCTATGTTGAAGCTACCAAACAGATCATCAAAACTGCTCGCTTCCTG AAGTCAGAACTGGAAAACATCAAAAACATCTTCATTTTCGGTGATCCTCAATTGTCAGTTATTGCTCTGG GATCCAACGATTTTGACATTTACCGACTATCTAATATGATGTCTGCTAAGGGGTGGAATTTTAACTACCT GCAGTTCCCAAGAAGCATTCATTTCTGCATTACGTTAGTACATACTCGGAAGCGAGTGGCGATCCAGTTC CTAAAGGATATCCGGGAATCAGTCACACAAATCATGAAGAATCCTAAAGCTAAGACCACAGGAATGGGTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4212293), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 53308. Forward Primer - name:053308_F_IRAV44_f10_Sgpl1, sequence:TACCGGGACTTGGCGTTA; Reverse Primer - name:053308_R_SP6_IRAV44_f10_Sgpl1, sequence:GCACCCATTCCTGTGGTC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8704 same embryo
	EMAGE:8706 same embryo
	EMAGE:8707 same embryo
	EMAGE:8705 same embryo
	EMAGE:8708 same embryo
	EurExpress:euxassay_009987 same experiment
	MGI:4828029 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source