EMAGE mouse gene expression spatial database

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EMAGE:8758

Cybrd1 cytochrome b reductase 1 ( MGI:2654575)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009953. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009953_01	euxassay_009953_02	euxassay_009953_03	euxassay_009953_04

euxassay_009953_05	euxassay_009953_06	euxassay_009953_07	euxassay_009953_08	euxassay_009953_09

euxassay_009953_10	euxassay_009953_11	euxassay_009953_12	euxassay_009953_13	euxassay_009953_14

euxassay_009953_15	euxassay_009953_16	euxassay_009953_17	euxassay_009953_18	euxassay_009953_19

euxassay_009953_20	euxassay_009953_21	euxassay_009953_22	euxassay_009953_23	euxassay_009953_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8758_wholemount_strong.wlz

8758_wholemount_moderate.wlz

8758_wholemount_possible.wlz

8758_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8758_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata alar plate ventricular layer

weak

regional

weak expression: see section 11 18 19

medulla oblongata basal plate ventricular layer

weak

regional

weak expression: see section 11 12 13 14 15

pons ventricular layer

weak

regional

weak expression: see section 08 09 10 11 14 15 16 17 18

midbrain ventricular layer

weak

regional

weak expression: see section 09 10 14 15

spinal cord ventricular layer

weak

regional

weak expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31079
Entity Detected:	Cybrd1, cytochrome b reductase 1 ( MGI:2654575)
Sequence:	sense strand is shown >T31079 TGATTGCGACGGTTCTCATGGGAGTGACAGAAAAGCTGTTCTTTGTCCTGAAACACCCCTCGTACCATTC ATTCCCACCAGAAGGTGTTTTTACAAATACGCTGGGCCTTCTGATTCTGGTGTTTGGAGCCCTCATCTTT TGGATTGTCACCAGGCCCCAATGGAAACGCCCTAGAGAACCAGGCTCTGTCCCTCTCCAGCTAAATGGAG GCAATGCAGAATGCAGAATGGAAGGCGCCATAGCAATAAGCTCCGCCCACAGTATGGACGCAGCTGACCC AGCTGACGCAGAGTCAAGCAGTGAGGGAGCAGCCCGGAAGAGAACCCTCGGCCTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:1243966), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 57216. Forward Primer - name:057216_F_IRAV40_g11_Cybrd1, sequence:TGATTGCGACGGTTCTCA; Reverse Primer - name:057216_R_SP6_IRAV40_g11_Cybrd1, sequence:CAAGGCCGAGGGTTCTCT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8756 same embryo
	EMAGE:8760 same embryo
	EMAGE:8757 same embryo
	EMAGE:8759 same embryo
	EMAGE:8755 same embryo
	EurExpress:euxassay_009953 same experiment
	MGI:4824150 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source