EMAGE mouse gene expression spatial database

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EMAGE:8761

Homer2 homer homolog 2 (Drosophila) ( MGI:1347354)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009975. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009975_01	euxassay_009975_02	euxassay_009975_03	euxassay_009975_04

euxassay_009975_05	euxassay_009975_06	euxassay_009975_07	euxassay_009975_08	euxassay_009975_09

euxassay_009975_10	euxassay_009975_11	euxassay_009975_12	euxassay_009975_13	euxassay_009975_14

euxassay_009975_15	euxassay_009975_16	euxassay_009975_17	euxassay_009975_18	euxassay_009975_19

euxassay_009975_20	euxassay_009975_21	euxassay_009975_22	euxassay_009975_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8761_wholemount_strong.wlz

8761_wholemount_moderate.wlz

8761_wholemount_weak.wlz

8761_wholemount_possible.wlz

8761_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8761_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 09 10 11 12 14 15 16 17

olfactory cortex marginal layer

strong

regional

strong expression: see section 09 10 11 12 14 15 16

facial vii ganglion

strong

regional

strong expression: see section 05 19

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 16 17 18 19 20 21

vagus x ganglion

strong

regional

strong expression: see section 16 17

ventral grey horn

strong

regional

strong expression: see section 09 10 12 13 14

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 14 15 16

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17

vomeronasal organ

strong

regional

strong expression: see section 11

left lung

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11

right lung

strong

regional

strong expression: see section 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31130
Entity Detected:	Homer2, homer homolog 2 (Drosophila) ( MGI:1347354)
Sequence:	sense strand is shown >T31130 TGGCACAGACGACGAAAAGGCCTCTCACGCGAGCCCAGCCGACACTCACCTCAAGTCTGAGAATGACAAG CTGAAGATCGCGCTGACACAGAGTGCTGCCAATGTGAAGAAGTGGGAGATGGAGCTGCAGACCCTGCGGG AGAGCAACGCCCGGCTGACCACGGCACTGCAGGAGTCGGCGGCCAGCGTGGAGCAGTGGAAGCGGCAGTT CTCCATCTGCAGGGACGAGAATGACAGGCTCCGCAGCAAGATCGAGGAGCTGGAAGAACAGTGCAGCGAG ATAAACAGGGAGAAGGAGAAGAACACACAGCTGAAGAGGAGGATCGAGGAGCTGGAGTCAGAGGTCCGAG ACAAGGAGATGGAGTTGAAAGATCTCCGAAAACAGAGTGAAATCATACCTCAGCTCATGTCCGAGTGTGA ATATGTCTCTGAGAAGTTAGAGGCGGCCGAAAGAGACAATCAAAACTTGGAAGACAAAGTGCGGTCTCTA AAGACAGACATCGAGGAGAGTAAATACCGACAGCGCCACCTGAAGGGGGAGCTGAAGAGCTTCCTTGAGG TGCTGGATGGAAAGATCGACGACCTCCATGACTTCCGTAGAGGACTCTCCAAGTTAGGCACAGATAACTA GGGCGGGGCGGAGCAAGTGTGTGTGAGAGGTGTGGTAGACGTAGGACATTCTCCATTTGCTTCTGTAAAT GCAGGTGCGATCTGTCTGTCTCCAGACCAATTGTGCCGTCCGCTCACTCCTCCAGAATAGGAAATCTCTC GCTTCTCTGGCTTTGTGAGGTCATGGACAGCTGGAAGCTTCTGACTCAGGAATCCAGAACTTGGTCTACC TTAGCCGTTTACGCAGTCAGGGCAGGGATGTTTAGATCTTCCCTTAAGGGCTGTTGTAACCCTATGAACC GGGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:1395337), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 57446. Forward Primer - name:057446_F_IRAV43_g04_Homer2, sequence:TGGCACAGACGACGAAAA; Reverse Primer - name:057446_R_SP6_IRAV43_g04_Homer2, sequence:TCCCCGGTTCATAGGGTT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8762 same embryo
	EMAGE:8763 same embryo
	EurExpress:euxassay_009975 same experiment
	MGI:4825406 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source