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EMAGE:880

Kdr kinase insert domain protein receptor ( MGI:96683)
TS11 (7.5 dpc)
in situ hybridisation

Data Images
EMAGE:880 EMAGE:880 EMAGE:880
Fig 2B Yamaguchi et al, 1993 [PMID:8223275] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig 2C Yamaguchi et al, 1993 [PMID:8223275] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig 2E Yamaguchi et al, 1993 [PMID:8223275] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Embryo B is neural plate stage (7.5dpc) and data from this embryo has been spatially mapped in this EMAGE entry. Embryos C and E are early headfold stage (7.75dpc) and are included here as examples of later TS11 embryos. A and C are viewed laterally, with anterior to the left, primitive streak to the right. Image annotations: a, allantois; e, endocardial tubes; nf, neural folds.
Expression Pattern Description
Spatial Annotation:
EMAGE:880Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
880_wholemount_strong_3D_1.wlz
880_wholemount_moderate_3D_1.wlz
880_wholemount_weak_3D_1.wlz
880_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:880_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
see Fig 2B
heart
strong strong
Labelled cells curve towards the midline to form the early endocardial tubes (Fig2E). Strong expression was seen within the endocardial tubes (Fig2C)
blood island
strong strong
see Fig 2c
allantois
strong strong
see Fig 2c
embryo mesoderm
strong strong
regionalExpression was strong in a band of embryonic mesodermal cells lateral to the primitive streak (Fig 2C)
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:14717
Entity Detected:Kdr, kinase insert domain protein receptor ( MGI:96683)
Sequence:sense strand is shown

>MGI:14717
TTGGCTCACAGGCAACATCGGTCCACATGGGCGAATCACTCACACCAGTTTGCAAGAACTTGGATGCTCT
TTGGAAACTGAATGGCACCATGTTTTCTAACAGCACAAATGACATCTTGATTGTGGCATTTCAGAATGCC
TCTCTGCAGGACCAAGGCGACTATGTTTGCTCTGCTCAAGATAAGAAGACCAAGAAAAGACATTGCCTGG
TCAAACAGCTCATCATCCTAGAGCGCATGGCACCCATGATCACCGGAAATCTGGAGAATCAGACAACAAC
CATTGGCGAGACCATTGAAGTGACTTGCCCAGCATCTGGAAATCCTACCCCACACATTACATGGTTCAAA
GACAACGAGACCCTGGTAGAAGATTCAGGCATTGTACTGAGAGATGGGAACCGGAACCTGACTATCCGCA
GGGTGAGGAAGGAGGATGGAGGCCTCTACACCTGCCAGGCCTGCAATGTCCTTGGCTGTGCAAGAGCGGA
GACGCTCTTCATAATAGAAGGTGCCCAGGAAAAGACCAACTTGGAAGTCATTATCCTCGTCGGCACTGCA
GTGATTGCCATGTTCTTCTGGCTCCTTCTTGTCATTCTCGTACGGACCGTTAAGCGGGCCAATGAAGGGG
AACTGAAGACAGGCTACTTGTCTATTGTCATGGATCCAGATGAATTGCCCTTGGATGAGCGCTGTGAACG
CTTGCCTTATGATGCCAGCAAGTGG
nt 1958 - nt 2682 of X59397.1
Notes:The Kdr (flk1) probe used in this study by Yamaguchi et al., 1993 [PMID:8223275] is described as "a partial flk-1 cDNA", "corresponding to nt 1958 to 2682 according to the nomenclature of Matthews et al, 1991 [PMID:1717995] . GenBank sequence X59397 is the same as appears in Matthews et al (in Fig 1).
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Strain:CD-1
Age:7.5 dpc
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Yamaguchi et al, 1993 [PMID:8223275] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:8223275] Yamaguchi TP, Dumont DJ, Conlon RA, Breitman ML, Rossant J 1993 flk-1, an flt-related receptor tyrosine kinase is an early marker for endothelial cell precursors. Development (118):489-98
 [ doi:10.1073/pnas.88.20.9026] [ PMID:1717995] Matthews W, Jordan CT, Gavin M, Jenkins NA, Copeland NG, Lemischka IR 1991 A receptor tyrosine kinase cDNA isolated from a population of enriched primitive hematopoietic cells and exhibiting close genetic linkage to c-kit. Proc Natl Acad Sci U S A (88):9026-30
Links:MGI:1277543 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI