Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:8809

Ubr5 ubiquitin protein ligase E3 component n-recognin 5 ( MGI:1918040)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8809 EMAGE:8809 EMAGE:8809 EMAGE:8809 EMAGE:8809
"Pseudo-wholemount" of euxassay_010003. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010003_01 euxassay_010003_02 euxassay_010003_03 euxassay_010003_04
EMAGE:8809 EMAGE:8809 EMAGE:8809 EMAGE:8809 EMAGE:8809
euxassay_010003_05 euxassay_010003_06 euxassay_010003_07 euxassay_010003_08 euxassay_010003_09
EMAGE:8809 EMAGE:8809 EMAGE:8809 EMAGE:8809 EMAGE:8809
euxassay_010003_10 euxassay_010003_11 euxassay_010003_12 euxassay_010003_13 euxassay_010003_14
EMAGE:8809 EMAGE:8809 EMAGE:8809 EMAGE:8809 EMAGE:8809
euxassay_010003_15 euxassay_010003_16 euxassay_010003_17 euxassay_010003_18 euxassay_010003_19
EMAGE:8809 EMAGE:8809 EMAGE:8809 EMAGE:8809 EMAGE:8809
euxassay_010003_20 euxassay_010003_21 euxassay_010003_22 euxassay_010003_23 euxassay_010003_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8809Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8809_wholemount_strong.wlz
8809_wholemount_moderate.wlz
8809_wholemount_weak.wlz
8809_wholemount_possible.wlz
8809_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8809_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
submandibular gland primordium
weak weak
regionalweak expression: see section 06 07 08 15 16 17 18
vibrissa
moderate moderate
regionalmoderate expression: see section 06 07 21 weak expression: see section 08 20
hypothalamus ventricular layer
moderate moderate
regionalmoderate expression: see section 14
diencephalon lateral wall ventricular layer
moderate moderate
regionalmoderate expression: see section 11 12 13 14
olfactory cortex ventricular layer
moderate moderate
regionalmoderate expression: see section 11 12 17
telencephalon ventricular layer
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 16 17 18 19 20 weak expression: see section 21 22 23
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 07 08 14 15
medulla oblongata basal plate ventricular layer
moderate moderate
regionalmoderate expression: see section 08 09 11 13 14
rest of cerebellum ventricular layer
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
pons mantle layer
moderate moderate
regionalmoderate expression: see section 07 weak expression: see section 16
pons ventricular layer
moderate moderate
regionalmoderate expression: see section 06 07 08 11 13 14 15 16 17
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 03 04 weak expression: see section 18 19
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 05 weak expression: see section 16
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 21 weak expression: see section 08 16 17 18 19 20
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 06 weak expression: see section 15
trigeminal v nerve
weak weak
regionalweak expression: see section 09 16
spinal cord ventricular layer
moderate moderate
regionalmoderate expression: see section 09 10
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 05 06 weak expression: see section 04 07 08 13 14 15
neural retina
weak weak
regionalweak expression: see section 01 02 03 23 24
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 09 10 11 12 14 15 16 17 18 weak expression: see section 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31769
Entity Detected:Ubr5, ubiquitin protein ligase E3 component n-recognin 5 ( MGI:1918040)
Sequence:sense strand is shown

>T31769
ATTGCTCATGGACGGGAAAATGGAGCTGACAGTATTTTGGACCTCGGATTATTAGACTCTTCAGAAAAGG
TACAGGAAAACAGAAAGCGCCATGGCTCTAGTAGAAGTGTAGTGGATATGGATTTAGAGGACACAGATGA
TGGTGATGACAACGCTCCCTTGTTTTACCAACCTGGGAAGAGAGGATTTTATACTCCGAGACCTGGCAAA
AACACAGAAGCAAGGCTGAATTGTTTCAGAAACATCGGCAGGATTCTTGGACTATGTCTGTTGCAAAATG
AACTATGTCCTATCACATTGAATAGGCATGTAATTAAAGTGTTGCTTGGTAGAAAAGTCAACTGGCATGA
CTTTGCTTTTTTCGACCCTGTAATGTATGAGAGTTTACGACAACTGATCCTTGCATCTCAGAGTTCAGAT
GCTGATGCTGTTTTCTCAGCAATGGATTTGGCATTTGCAATTGACCTGTGTAAAGAAGAGGGTGGAGGAC
AGGTTGAACTTATTCCAAATGGTGTAAATATACCAGTCACTCCGCAGAATGTATATGAGTATGTAAGGAA
ATATGCAGAGCATAGAATGTTGGTGGTTGCAGAACAGCCATTGCACGCAATGAGAAAGGGTCTACTAGAC
GTGCTTCCAAAAAATTCCTTAGAAGACTTAACAGCTGAGGACTTCCGGCTCTTGGTGAACGGCTGTGGTG
AAGTCAATGTGCAGATGCTGATCAGTTTCACTTCTTTCAATGATGAGTCAGGAGAAAATGCTGAGAAGCT
CCTCCAGTTCAAACGGTGGTTCTGGTCAATAGTAGAGAAAATGAGCATGACAGAACGGCAGGACCTGGTT
TACTTCTGGACATCTAGCCCATCATTGCCAGCCAGTGAAGAAGGTTTTCAGCCTATGCCCTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4481326), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59424. Forward Primer - name:059424_F_IRAV96_g03_4432411E13Rik, sequence:ATTGCTCATGGACGGGAA; Reverse Primer - name:059424_R_SP6_IRAV96_g03_4432411E13Rik, sequence:CGAGGGCATAGGCTGAAA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8808 same embryo
 EurExpress:euxassay_010003 same experiment
 MGI:4829066 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS