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EMAGE:8821

Krt15 keratin 15 ( MGI:96689)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8821 EMAGE:8821 EMAGE:8821 EMAGE:8821 EMAGE:8821
"Pseudo-wholemount" of euxassay_010051. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010051_01 euxassay_010051_02 euxassay_010051_03 euxassay_010051_04
EMAGE:8821 EMAGE:8821 EMAGE:8821 EMAGE:8821 EMAGE:8821
euxassay_010051_05 euxassay_010051_06 euxassay_010051_07 euxassay_010051_08 euxassay_010051_09
EMAGE:8821 EMAGE:8821 EMAGE:8821 EMAGE:8821 EMAGE:8821
euxassay_010051_10 euxassay_010051_11 euxassay_010051_12 euxassay_010051_13 euxassay_010051_14
EMAGE:8821 EMAGE:8821 EMAGE:8821 EMAGE:8821 EMAGE:8821
euxassay_010051_15 euxassay_010051_16 euxassay_010051_17 euxassay_010051_18 euxassay_010051_19
EMAGE:8821 EMAGE:8821 EMAGE:8821 EMAGE:8821 EMAGE:8821
euxassay_010051_20 euxassay_010051_21 euxassay_010051_22 euxassay_010051_23 euxassay_010051_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8821Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8821_wholemount_strong.wlz
8821_wholemount_moderate.wlz
8821_wholemount_weak.wlz
8821_wholemount_possible.wlz
8821_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8821_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
moderate moderate
regionalmoderate expression: see section 12 13 14 15 weak expression: see section 16
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 07 08 09 10 17 18 19 20
epidermis
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
vibrissa
strong strong
regionalstrong expression: see section 03 04 05 06 07 18 19 20 21 22
pharyngo-tympanic tube
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 18 19 20 21 22 23 24
cornea epithelium
strong strong
regionalstrong expression: see section 01 02 03 04 22 23 24
naris
strong strong
regionalstrong expression: see section 10 11 12 13 14 15
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 11 14 15 moderate expression: see section 08 09 10 16 17 18
esophagus
strong strong
regionalstrong expression: see section 12 13 14 15 16
stomach
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08
rectum
moderate moderate
regionalmoderate expression: see section 12
midgut
weak weak
regionalweak expression: see section 11 12 13 14 15 16 17 18 19
lower jaw incisor
strong strong
regionalstrong expression: see section 10 11 12 13 14 15 16 17
lower jaw molar
strong strong
regionalstrong expression: see section 08 17 18 19
oral epithelium
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
palatal shelf epithelium
strong strong
regionalstrong expression: see section 10 11 12 13 14 15 16 17
upper jaw incisor
strong strong
regionalstrong expression: see section 11 14 15
upper jaw molar
strong strong
regionalstrong expression: see section 08 19
bladder
strong strong
regionalExpression in the fundus region.
urethra of male
strong strong
regionalstrong expression: see section 11 12 13
larynx
strong strong
regionalstrong expression: see section 13 14 15
trachea
strong strong
regionalstrong expression: see section 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31813
Entity Detected:Krt15, keratin 15 ( MGI:96689)
Sequence:sense strand is shown

>T31813
GGTGATTTTGGTGGTGGCGATGGAGGTCTCCTCTCTGGCAATGAGAAGGTGACCATGCAGAACCTTAATG
ACCGCCTGGCCTCCTACCTGGACAAGGTGCGTGCCCTAGAGCAGGCCAACACTGAGCTGGAGGTGAAGAT
CCGAGATTGGTACCAGAAGCAGAGCCCAGCCAGTCCAGATCGGGACTACAGCCATTACTTTAAGACCATG
GAAGAGATCCGGGACAAAATTCTGGCTGCCACCATTGACAACTCGCGCGTTGTCCTGGAGATTGACAATG
CCAGGCTGGCGGCGGACGACTTCAGGCTCAAGTATGAGAATGAGCTGACCCTGCGTCAGGGCGTGGAGGC
CGACATCAACGGGCTGCGCCGGGTGCTGGATGAGCTGACCCTGGCCAGGACCGACCTGGAGATGCAGATT
GAGCAGCTGAACGAGGAGCTGGCTTACCTGAAGAAGAACCATGAGGAGGAGATGAAGGAATTCAGCAGTC
AACTGGCTGGCCAGGTCAATGTGGAAATGGACGCAGCACCCGGGGTGGACCTGACCCGCATGCTGGCAGA
GATGAGGGAGCAGTATGAAGCTATTGCAGAGAAAAACCGTCGGGATGTAGAGGCCTGGTTCTTCAGTAAG
ACTGAAGAGCTGAACAAGGAGGTGGCGTCTAACACAGAAATGATCCAGACCAGCAAGACGGAGATCACAG
ACCTGAGACGGACCCTGCAGGGGCTAGAGATTGAGTTGCAGTCTCAGCTCAGCATGAAAGCCGGGCTGGA
GAACTCTCTGGCAGAGGTGGAGTGCCGCTATGCCACACAGCTGCAGCAGATCCAGGGGGTCATTACCGGT
CTGGAGACTCAGCTGAGTGAGCTCCGCTGTGAGATGGAGGCTCAGAACCAGGAGTACAACATGCTGCTGG
ACATCAAGACTCGGCTGGAACAGGAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5251453), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 60257. Forward Primer - name:060257_F_IRAV99_a09_Krt1-15, sequence:GGTGATTTTGGTGGTGGC; Reverse Primer - name:060257_R_SP6_IRAV99_a09_Krt1-15, sequence:TCTCCTGTTCCAGCCGAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8825 same embryo
 EMAGE:8826 same embryo
 EMAGE:8824 same embryo
 EMAGE:8823 same embryo
 EMAGE:8822 same embryo
 EurExpress:euxassay_010051 same experiment
 MGI:4825828 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS