EMAGE mouse gene expression spatial database

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EMAGE:8823

Serpinb6a serine (or cysteine) peptidase inhibitor, clade B, member 6a ( MGI:103123)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010066. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010066_01	euxassay_010066_02	euxassay_010066_03	euxassay_010066_04

euxassay_010066_05	euxassay_010066_06	euxassay_010066_07	euxassay_010066_08	euxassay_010066_09

euxassay_010066_10	euxassay_010066_11	euxassay_010066_12	euxassay_010066_13	euxassay_010066_14

euxassay_010066_15	euxassay_010066_16	euxassay_010066_17	euxassay_010066_18	euxassay_010066_19

euxassay_010066_20	euxassay_010066_21	euxassay_010066_22	euxassay_010066_23	euxassay_010066_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8823_wholemount_strong.wlz

8823_wholemount_moderate.wlz

8823_wholemount_weak.wlz

8823_wholemount_possible.wlz

8823_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8823_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

weak

regional

weak expression: see section 07 08 09 10 11 12 13 16 17 18 19 20 21

mesenchyme

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

adrenal gland

strong

regional

strong expression: see section 07 08 09 15 16 17 weak expression: see section 18

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 11 18 19 20 21

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 10 11 17 18

pons mantle layer

weak

regional

weak expression: see section 09 19

facial vii ganglion

weak

regional

weak expression: see section 06 07 22 23

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 09 moderate expression: see section 08 20 weak expression: see section 21

trigeminal v ganglion

weak

regional

weak expression: see section 05 06 07 08 09 10 18 19 20 21 22 23 24

vagus x ganglion

strong

regional

strong expression: see section 10 19

ventral grey horn

weak

regional

weak expression: see section 11 12 14 15 16

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 14 15 16 17 18 19 20

hindgut

moderate

regional

moderate expression: see section 13 14

rectum

moderate

regional

moderate expression: see section 12 13

midgut

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 19 20

testis

strong

regional

strong expression: see section 05 06 07 08 18 19 20 21

trachea

weak

regional

weak expression: see section 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31809
Entity Detected:	Serpinb6a, serine (or cysteine) peptidase inhibitor, clade B, member 6a ( MGI:103123)
Sequence:	sense strand is shown >T31809 GGGGCAAAAGGAACCACTGCTAGCCAGATGGCTCAGGCACTTGCTTTGGATAAATGCAGCGGCAATGGAG GTGGAGATGTCCACCAGGGCTTCCAGTCCCTTCTCACCGAAGTGAACAAAACTGGCACACAGTACTTGCT CAGAACAGCCAACAGGCTCTTCGGGGATAAGACTTGTGATCTTTTAGCGTCTTTTAAAGATTCCTGCCTC AAGTTCTATGAAGCAGAGTTGGAAGAGCTGGACTTTCAGGGTGCTACAGAGGAGTCCCGACAGCACATCA ACACCTGGGTAGCCAAAAAGACAGAAGATAAAATCAAAGAGGTGCTGTCTCCAGGTACAGTGAATTCTGA TACATCGCTAGTCCTTGTGAATGCCATCTACTTCAAAGGAAACTGGGAGAAGCAGTTTAACAAAGAGCAT ACCAGGGAGATGCCATTCAAAGTCAGCAAGAATGAGGAGAAACCTGTGCAAATGATGTTTAAGAAGTCTA CCTTTAAGATGACCTATATTGGAGAGATATTCACTAAGATTCTGTTGCTTCCCTATGTCAGCAGTGAGCT GAACATGATCATCATGCTTCCAGATGAGCACGTTGAACTGAGTACAGTGGAAAAGGAAGTAACTTACGAG AAATTTATAGAGTGGACAAGGCTGGACAAGATGGACGAAGAAGAGGTAGAAGTATTTCTCCCAAAGTTTA AGCTGGAGGAGAATTACAACATGAACGATGCCCTCTACAAGTTGGGCATGACTGATGCCTTTGGCGGCAG GGCAGACTTTTCTGGAATGTCTTCCAAGCAAGGCTTGTTTCTGTCTAAGGTTGTGCATAAGGCCTTTGTG GAGGTTAATGAGGAGGGCACAGAGGCTGCAGCTGCTACAGCTGGCATGATGACGGTGAGGTGCATGAGAT TCACTCCCCGCTTCTGTGCCGACCACCCCTTCCTTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5342439), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 60284. Forward Primer - name:060284_F_IRAV98_g12_Serpinb6a, sequence:GGGGCAAAAGGAACCACT; Reverse Primer - name:060284_R_SP6_IRAV98_g12_Serpinb6a, sequence:AAAAGGAAGGGGTGGTCG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8825 same embryo
	EMAGE:8821 same embryo
	EMAGE:8826 same embryo
	EMAGE:8824 same embryo
	EMAGE:8822 same embryo
	EurExpress:euxassay_010066 same experiment
	MGI:4827993 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source