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EMAGE:8825

Hdhd2 haloacid dehalogenase-like hydrolase domain containing 2 ( MGI:1924237)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8825 EMAGE:8825 EMAGE:8825 EMAGE:8825 EMAGE:8825
"Pseudo-wholemount" of euxassay_010047. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010047_01 euxassay_010047_02 euxassay_010047_03 euxassay_010047_04
EMAGE:8825 EMAGE:8825 EMAGE:8825 EMAGE:8825 EMAGE:8825
euxassay_010047_05 euxassay_010047_06 euxassay_010047_07 euxassay_010047_08 euxassay_010047_09
EMAGE:8825 EMAGE:8825 EMAGE:8825 EMAGE:8825 EMAGE:8825
euxassay_010047_10 euxassay_010047_11 euxassay_010047_12 euxassay_010047_13 euxassay_010047_14
EMAGE:8825 EMAGE:8825 EMAGE:8825 EMAGE:8825 EMAGE:8825
euxassay_010047_15 euxassay_010047_16 euxassay_010047_17 euxassay_010047_18 euxassay_010047_19
EMAGE:8825 EMAGE:8825 EMAGE:8825 EMAGE:8825 EMAGE:8825
euxassay_010047_20 euxassay_010047_21 euxassay_010047_22 euxassay_010047_23 euxassay_010047_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8825Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8825_wholemount_strong.wlz
8825_wholemount_moderate.wlz
8825_wholemount_weak.wlz
8825_wholemount_possible.wlz
8825_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8825_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31812
Entity Detected:Hdhd2, haloacid dehalogenase-like hydrolase domain containing 2 ( MGI:1924237)
Sequence:sense strand is shown

>T31812
GGATGGAGCCCCTCTGATAGCTATCCACAAGGCCAGGTACTACAAGAGAAAAGATGGCTTAGCCTTGGGA
CCAGGACCATTTGTGACTGCCTTAGAGTATGCCACAGACACGAAAGCCATGGTGGTGGGGAAACCAGAGA
AGACATTCTTTCTGGAAGCCTTGAGGGATGCTGACTGTGCACCGGAGGAGGCCGTCATGATAGGAGACGA
TTGCAGGGATGATGTCGATGGGGCTCAGAACATTGGCATGCTGGGCATCTTAGTTAAAACCGGGAAATAC
AAAGCAGCAGATGAAGAAAAAATTAATCCACCTCCCTACTTAACTTGCGAGAGCTTCCCTCACGCTGTGG
ACCACATTCTGCAGCACCTCCTGTGAACCAGCATGTGTTGTCTGGACTGGATGGATCCCTAACAAGGTGG
CATCAGTAGGCTGCGCCATCCAGTGCTGATCCTATTTAACCCTTTCATTGTGCATTAATTAGAAAGAGGG
GGATTGGATTGCAGCCAACCCTAAACCTTGCTAATCTCTTTTGTTTTATAAAAGAAGATGGGACCTGAAG
AAAGGGAGAGCTGAGATTTTGTGCCATTCCTGTTAAAATATTCATCAGGTTCACTGGGCTGGAGGAGAAG
CTCCTGTGGGCGCAGAGGAGCAGTAGCTCTCCACTCCTCCGTGGTGGAAGTGAGAGGATCCTCTGTGAGC
ATGACTGACTCACACAGAGATGCACTGGAAGGTGCATTAGGCTGTCAGCAAGATGCCAGAGTTGAGCCGT
GAAGCTCTGCAGACAAGCCACAGAGAAGCAAGCTAGCAGCAGTGTGGCGA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5250739), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59407. Forward Primer - name:059407_F_IRAV99_a08_3110052N05Rik, sequence:GGATGGAGCCCCTCTGAT; Reverse Primer - name:059407_R_SP6_IRAV99_a08_3110052N05Rik, sequence:CTCGCCACACTGCTGCTA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8821 same embryo
 EMAGE:8826 same embryo
 EMAGE:8824 same embryo
 EMAGE:8823 same embryo
 EMAGE:8822 same embryo
 EurExpress:euxassay_010047 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS