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EMAGE:8835

Olfr424 olfactory receptor 424 ( MGI:3030258)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8835 EMAGE:8835 EMAGE:8835 EMAGE:8835 EMAGE:8835
"Pseudo-wholemount" of euxassay_013042. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013042_01 euxassay_013042_02 euxassay_013042_03 euxassay_013042_04
EMAGE:8835 EMAGE:8835 EMAGE:8835 EMAGE:8835 EMAGE:8835
euxassay_013042_05 euxassay_013042_06 euxassay_013042_07 euxassay_013042_08 euxassay_013042_09
EMAGE:8835 EMAGE:8835 EMAGE:8835 EMAGE:8835 EMAGE:8835
euxassay_013042_10 euxassay_013042_11 euxassay_013042_12 euxassay_013042_13 euxassay_013042_14
EMAGE:8835 EMAGE:8835 EMAGE:8835 EMAGE:8835 EMAGE:8835
euxassay_013042_15 euxassay_013042_16 euxassay_013042_17 euxassay_013042_18 euxassay_013042_19
EMAGE:8835 EMAGE:8835 EMAGE:8835 EMAGE:8835 EMAGE:8835
euxassay_013042_20 euxassay_013042_21 euxassay_013042_22 euxassay_013042_23 euxassay_013042_24
EMAGE:8835 EMAGE:8835
euxassay_013042_25 euxassay_013042_26

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8835Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8835_wholemount_strong.wlz
8835_wholemount_moderate.wlz
8835_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8835_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39127
Entity Detected:Olfr424, olfactory receptor 424 ( MGI:3030258)
Sequence:sense strand is shown

>T39127
TGGCTATTGACAGGTATGTTGCCATCTGTAATCCTCTCCGGTACCCAACTATCATGACTCCCCGGCTCTG
TGCTCAGCTCTCTGCAGGCTCCTGCATCTTTGGCTTTCTTGTGTTGCTTCCAGAGATTGCTTGGATTTCC
ACACTGCCCTTCTGTGGCCCCAACCAAATTCATCAGATCTTCTGTGACTTTGAACCTGTGTTGCGTTTGG
CATGTACGGACACCTCCATGATTCTGGTTGAAGATGTGGTTCATGCAGTGGCCATCATCTTCTCTGTGTT
GGTTATTGCCATCTCTTACATGAGAATCATCACCGTGATCCTGAGGATCCCCTCTGGTGAAGGCCGTCGG
AAGGCTTTCTCTACTTGTGCTGCCCATCTTGGTGTCTTCTTGATGTTCTATGGCAGTGTCTCCCTCATGT
ATCTGCGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 152412. Forward Primer - name:152412_F_cDNA_Olfr424, sequence:TGGCTATTGACAGGTATGTTGC; Reverse Primer - name:152412_N_SP6_cDNA_Olfr424, sequence:ACGCAGATACATGAGGGAGAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8834 same embryo
 EMAGE:8836 same embryo
 EMAGE:8831 same embryo
 EMAGE:8833 same embryo
 EMAGE:8832 same embryo
 EurExpress:euxassay_013042 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS