EMAGE mouse gene expression spatial database

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EMAGE:8860

Iqgap1 IQ motif containing GTPase activating protein 1 ( MGI:1352757)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010153. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010153_01	euxassay_010153_03	euxassay_010153_04	euxassay_010153_05

euxassay_010153_06	euxassay_010153_07	euxassay_010153_08	euxassay_010153_09	euxassay_010153_10

euxassay_010153_11	euxassay_010153_12	euxassay_010153_13	euxassay_010153_14	euxassay_010153_15

euxassay_010153_16	euxassay_010153_18	euxassay_010153_19	euxassay_010153_20	euxassay_010153_21

euxassay_010153_22	euxassay_010153_23	euxassay_010153_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8860_wholemount_strong.wlz

8860_wholemount_moderate.wlz

8860_wholemount_weak.wlz

8860_wholemount_possible.wlz

8860_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8860_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

weak

regional

weak expression: see section 07 21 22

lateral ventricle choroid plexus

weak

regional

weak expression: see section 06 07 08 09 10 13 14 15 16 17 18 19 20

metencephalon part of 4th ventricle choroid plexus

weak

regional

weak expression: see section 04 05 07 08 09 10 13 14 15 16 17 18 19 20

nasal cavity epithelium

moderate

regional

moderate expression: see section 13 14 weak expression: see section 16 17 18 19

hindgut

weak

regional

weak expression: see section 11

midgut

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 weak expression: see section 10 11 12 20

mandible

weak

regional

weak expression: see section 03 04 05 06 07 08 21 22 23

oral epithelium

moderate

regional

moderate expression: see section 19 20 21

metanephros

moderate

regional

moderate expression: see section 05 06 07 08 09 15 16 17 18

lung

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 13 14 15 16 17 18 19 20 21 22 23 24 weak expression: see section 11

orbito-sphenoid

weak

regional

weak expression: see section 02 03 04 05 06 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T32078
Entity Detected:	Iqgap1, IQ motif containing GTPase activating protein 1 ( MGI:1352757)
Sequence:	sense strand is shown >T32078 AGTTCCCTGACGCTGGTGAGGACGAGCTGCTGAAGATTATCGGTAACCTGCTTTACTACCGATACATGAA CCCAGCCATCGTCGCTCCCGATGCCTTCGACATCATTGACCTGTCAGCAGGGGGCCAGCTCACCACAGAC CAGCGCAGAAACCTGGGCTCCATTGCCAAGATGCTCCAGCACGCGGCGTCCAACAAGATGTTTCTGGGCG ATAATGCCCACTTAAGCATCATTAATGAGTATCTCTCGCAGTCCTACCAGAAATTCAGACGGTTTTTCCA ATTGGCTTGTGACGTCCCAGAGCTGCAGGATAAATTTAACGTGGATGAGTACTCTGACCTAGTCACCCTC ACTAAGCCAGTTATCTACATCTCCATTGGCGAAATCATCAACACCCACACTCTCCTGTTGGACCATCAGG ATGCCATTGCTCCAGAGCATAACGACCCCATCCACGAACTTCTGGACGACCTTGGGGAGGTGCCCACCAT TGAGTCCCTTATAGGAGAAAGCTGTGGCAATTCAAACGACCCCAACAAGGAGGCTCTGGCTAAGACGGAA GTGTCTCTCACGTTGACCAACAAGTTTGACGTGCCTGGTGACGAGAACGCAGAGATGGACGCTCGGACCA TCTTACTGAATACAAAACGTTTAATTGTGGATGTCATCCGGTTCCAGCCAGGAGAGACCTTGACTGAAAT TCTAGAAACCCCAGCCACCAATGAACAGGAAGCTGAACATCAGAGGGCCATGCAGAGACGGGCTATCCGC GATGCCAAAACCCCTGACAAGATGAAAAAATCAAAGCCCATGAAGGAGGATAACAACCTCAGCCTCCAGG AGAAGAAAGAGAAGATCCAGACTGGCCTAAAGAAGCTAACGGAGCTTGGGACGGTGGACCCAAAGAACAG ATACCAGGAACTCATCAACGACATTGCCAAGGATATCCGGA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3256630), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 57950. Forward Primer - name:057950_F_IRAV89_h11_Iqgap1, sequence:AGTTCCCTGACGCTGGTG; Reverse Primer - name:057950_R_SP6_IRAV89_h11_Iqgap1, sequence:TTCCGGATATCCTTGGCA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8857 same embryo
	EMAGE:8858 same embryo
	EMAGE:8859 same embryo
	EMAGE:8856 same embryo
	EMAGE:8855 same embryo
	EurExpress:euxassay_010153 same experiment
	MGI:4825613 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source