EMAGE mouse gene expression spatial database

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EMAGE:888

Hoxb8 homeobox B8 ( MGI:96189)
TS11 (Early headfold Downs & Davies)
in situ hybridisation

Data Images


Figure 1G of Forlani et al., 2003 [PMID:12835396] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright.	Figure 1H of Forlani et al., 2003 [PMID:12835396] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright.	Figure 1I of Forlani et al., 2003 [PMID:12835396] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright.	Figure 1J of Forlani et al., 2003 [PMID:12835396] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

All specimens shown are TS11 and text annotation in this EMAGE entry refers to all of these samples collectively but only the data from specimen H has been spatially mapped in this EMAGE entry as this sample morphologically matches the EMAP TS11 model (#1). Embryo stages (Downs and Davies staging) are: (G) late neural plate (LNP); (H,I) early headfold (EHF); (J) late headfold (LHF). Image annotations: Line in (G) = extent of the primitive streak; arrowheads = rostral front of expression; n, node. Anterior is towards the left; posterior towards the right.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

888_wholemount_strong_3D_1.wlz

888_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:888_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo mesoderm

detected

regional

Transcripts remained mainly restricted to the primitive streak and nascent mesoderm.

primitive streak

detected

regional

Transcripts remained mainly restricted to the primitive streak and nascent mesoderm. Transcripts reached the anterior end of the streak.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:1339294
Entity Detected:	Hoxb8, homeobox B8 ( MGI:96189)
Sequence:	sense strand is shown >MGI:1339294 TCGGCCCCGCGAGCGACGCAGGAGCTGGGCCTCCCACAGCAGCGTCCCCCGCCGCGCCAGTCCCCGCTAG TGGTAGTATCTCGTAATAGCTTCTGTGTGTGAGCTACCGTGGATCTCCTTCCCTTGCTTCTGTGTGTGAG CTACCGTGGATCTCCTTCCCTTCTCTTGGGGGTCCGGGGGGAAAAAAAGAAAAGGATTTTAAGCAAGGAC TCTTAAGCAAGGACTCCCTCGTCCTGCGAGGGTGATCGACTGCGGCCTGGCAGAACCCCCTCGCCCCCGC CCCATGTAAAAAAGCCTCCTTGTGCAATGGTCTGTTTCCTTTGAACGTGCTTCTTTGTAATGACCGAGGT AC
	nt 1886 - nt 2237 of NM_010461.1
Notes:	The probe used in study by Forlani et al., 2003 [PMID:12835396] is described as a "1:1 mix of a SP6 polymerase transcript from a 350 bp 3' untranslated SacI-KpnI cDNA fragment and a SP6 polymerase transcript from a 420 bp SacI fragment containing the first exon of the gene (Charit� et al., 1994 [PMID:7915198] )". The GenBank ID and co-ordinates above indicating the sequence refer only to the first of these probes.
Chemistry:	RNA
Strand:	antisense

Specimen

Organism:	mouse
Strain:	(C57BL/6J x CBA)F1
Age:	Early headfold Downs & Davies
Theiler Stage:	TS11
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

General Information

Authors:	Forlani et al., 2003 [PMID:12835396] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1242/dev.00573] [ PMID:12835396] Forlani S, Lawson KA, Deschamps J 2003 Acquisition of Hox codes during gastrulation and axial elongation in the mouse embryo. Development (130):3807-19
	[ doi:10.1016/0092-8674(94)90524-X] [ PMID:7915198] Charite J, de Graaff W, Shen S, Deschamps J 1994 Ectopic expression of Hoxb-8 causes duplication of the ZPA in the forelimb and homeotic transformation of axial structures. Cell (78):589-601
Links:	MGI:2668657 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source