EMAGE mouse gene expression spatial database

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EMAGE:8948

Ccdc36 coiled-coil domain containing 36 ( MGI:3612242)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012895. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012895_01	euxassay_012895_02	euxassay_012895_03	euxassay_012895_04

euxassay_012895_05	euxassay_012895_06	euxassay_012895_07	euxassay_012895_08	euxassay_012895_09

euxassay_012895_10	euxassay_012895_11	euxassay_012895_12	euxassay_012895_13	euxassay_012895_14

euxassay_012895_15	euxassay_012895_16	euxassay_012895_17	euxassay_012895_18	euxassay_012895_19

euxassay_012895_20	euxassay_012895_21	euxassay_012895_22	euxassay_012895_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8948_wholemount_strong.wlz

8948_wholemount_moderate.wlz

8948_wholemount_weak.wlz

8948_wholemount_possible.wlz

8948_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8948_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

ventral grey horn

weak

single cell

weak expression: see section 12 13 16 17

mandible

moderate

single cell

moderate expression: see section 03 04 05 06 07 08 09 10 14 15 16 17 18 19 20 22 weak expression: see section 21

meckel's cartilage

moderate

single cell

moderate expression: see section 18 19 weak expression: see section 06 07 08 09 10 11 13 14 15 16 17

maxilla

moderate

single cell

moderate expression: see section 03 04 05 06 07 16 17 18 19 20 weak expression: see section 21

lung

moderate

single cell

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 21 22 23 weak expression: see section 20

temporal bone petrous part

moderate

regional

moderate expression: see section 02 03 05 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38685
Entity Detected:	Ccdc36, coiled-coil domain containing 36 ( MGI:3612242)
Sequence:	sense strand is shown >T38685 CTCACTTGAACAGTCAGCAACCGAACAGAAAGGCAGGCCCTGCAGAAAAAGGAGGAGAGGCAAGAAGCAA CAACCCCAGAGATCAAAGAGAGGTGGGCTTCTAGATAGGAAGCAAGGACAGACCTCAAAGGCGGCCTGTG CTTTCATAGCTAGGCATCACTGCCCCCAGTCTCCAGTGTGTGATCCACAGGGGCCCCTCATCTGTTGGCT GACTCCGAGAAGTTCCACAAAGTCAACCTGTCACATTTTGGGAGGAACGGGAGAGACCAGTCAGACAGCA AGGGCAGCACAAGGGAACCTCGTGCAGCATTCCCAGCGCTCTTCCACAGACAGCAGCTCCCAGGGAGACC AGCAGATAAATTGGTTCAGTGACCTCAGCCTTGAAAACCTAGAGCCGCCACAGTGTAAGAAGGGAGGGAC GAATTTGCTCTGTGACCCGGATTTTGATAGCAGCGATGATAATTTCTAACCTGCCAGCAGTGACTTTAGC TGATGGTTTGTTATCTCAGAACGACAAACTGTAGGACATTGGATTGAGCAACAGCACACAGCATCTGAAG CTGGCTTATCCAGCCTCAGAATCAGAGGCCTTGACTGACTGTGGCCAGTAGGAGGAAGGGGAGAATCCAG TACTTTCAGTATCAGTTGCTGCTCACAGCGGGAAGTTACGGGGAAGAAATGTGTGGAGACAGTGAAGAGA ACTCAGGGAGTAGCCTGTGGTGAGGCATCAGAAAGTGGCTGTATGTCCTTGGTGAAAACATTGGCATTGG CTTCCTTACGGGATACAATTTAGAAGAACTCTCAGAAACTTTGTGACTGTGTGAGTAGCTCTCAAGACGT TCCCTGCACCTGACTGGTACCTTCCTGAGCCTCAGCTTTATCTTTACCTAGTGCTGTCAAAGAGTTTTGT CTTTTCCCTGAGAGGTCATGTTACAGGGTACACT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 109417. Forward Primer - name:109417_F_cDNA_LOC434438, sequence:CTCACTTGAACAGTCAGCAACC; Reverse Primer - name:109417_N_SP6_cDNA_LOC434438, sequence:AGTGTACCCTGTAACATGACCTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8950 same embryo
	EMAGE:8947 same embryo
	EMAGE:8949 same embryo
	EMAGE:8951 same embryo
	EMAGE:8946 same embryo
	EurExpress:euxassay_012895 same experiment
	MGI:4823675 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source