Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:895

Lfng LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase ( MGI:1095413)
TS11 (7.5 dpc)
in situ hybridisation

Data Images
EMAGE:895
Fig 4I Przemeck et al, 2003 [PMID:12441287] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Top image: view towards to the distal tip of the embryo. Bottom image: lateral view of the same embryo. Image annotations: red circle = approximate position of the node; asterisk = position of the allantois.
Expression Pattern Description
Spatial Annotation:
EMAGE:895Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
895_wholemount_strong_3D_1.wlz
895_wholemount_moderate_3D_1.wlz
895_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:895_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
regionalExpression in a distinct domain lateral to the node.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2445977
Entity Detected:Lfng, LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase ( MGI:1095413)
Sequence:sense strand is shown

>MGI:2445977
TCCAGGGCGGCTGTGGATCCCGGAGTCCTCACCCGCGAGGTGCATAGCCTCTCCGAGTACTTCAGTCTAC
TCACCCGCGCGCGCAGAGACGCGGATCCACCGCCCGGGGTCGCTTCTCGCCAGGGCGACGGCCATCCGCG
TCCCCCCGCCGAAGTTCTGTCCCCTCGCGACGTCTTCATCGCCGTCAAGACCACCAGAAAGTTTCACCGC
GCGCGGCTCGATCTGCTGTTCGAGACCTGGATCTCGCGCCACAAGGAGATGACGTTCATCTTCACTGATG
GGGAGGACGAAGCTCTGGCCAAGCTCACAGGCAATGTGGTGCTCACCAACTGCTCCTCGGCCCACAGCCG
CCAGGCTCTGTCCTGCAAGATGGCTGTGGAGTATGACCGATTCATTGAGTCTGGGAAGAAGTGGTTCTGC
CACGTGGATGATGACAACTACGTCAACCTCCGGGCGCTGCTGCGGCTCCTGGCCAGCTATCCCCACACCC
AAGACGTGTACATCGGCAAGCCCAGCCTGGACAGGCCCATCCAGGCCACAGAACGGATCAGCGAGCACAA
AGTGAGACCTGTCCACTTTTGGTTTGCCACCGGAGGAGCTGGCTTCTGCATCAGCCGAGGGCTGGCCCTA
AAGATGGGCCCATGGGCCAGTGGAGGACACTTCATGAGCACGGCAGAGCGCATCCGGCTCCCCGATGACT
GCACCATTGGCTACATTGTAGAGGCTCTGCTGGGTGTACCCCTCATCCGGAGCGGCCT
nt 273 - nt 1030 of AF015768.1
Notes:The probe used in this study by Przemeck et al, 2003 [PMID:12441287] is indicated as that described by Cohen et al, 1997 [PMID:9207795] ie. "A probe for lunatic fringe was generated by PCR with the use of the following primers: 5'-GAATTC CTG CTG TTC GAG ACC TGG ATC (contains EcoRI site) and 5'-AGATCT ACC AGG ATT GTA GAA GAT CGC (contains BglII site)" and pBK-lunatic (clone 24) as template. The 756-bp PCR product", "spans nt 273 to nt 1030 of the lunatic coding sequence". AF015768.1 is the accompanying nucleotide sequence submission to GenBank from Cohen et al.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:129S1/Sv x129X1/SvJ x C57BL/6J
Age:7.5 dpc
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Przemeck et al, 2003 [PMID:12441287] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.00176] [ PMID:12441287] Przemeck GK, Heinzmann U, Beckers J, Hrabe de Angelis M 2003 Node and midline defects are associated with left-right development in Delta1 mutant embryos. Development (130):3-13
 [ doi:10.1038/ng0797-283] [ PMID:9207795] Cohen B, Bashirullah A, Dagnino L, Campbell C, Fisher WW, Leow CC, Whiting E, Ryan D, Zinyk D, Boulianne G, Hui CC, Gallie B, Phillips RA, Lipshitz HD, Egan SE 1997 Fringe boundaries coincide with Notch-dependent patterning centres in mammals and alter Notch-dependent development in Drosophila. Nat Genet (16):283-8
Links:MGI:2449023 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI