EMAGE mouse gene expression spatial database

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EMAGE:8959

Cp ceruloplasmin ( MGI:88476)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012898. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012898_01	euxassay_012898_02	euxassay_012898_03	euxassay_012898_04

euxassay_012898_05	euxassay_012898_06	euxassay_012898_07	euxassay_012898_08	euxassay_012898_09

euxassay_012898_10	euxassay_012898_11	euxassay_012898_12	euxassay_012898_13	euxassay_012898_14

euxassay_012898_15	euxassay_012898_16	euxassay_012898_17	euxassay_012898_18	euxassay_012898_19

euxassay_012898_20	euxassay_012898_21	euxassay_012898_22	euxassay_012898_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8959_wholemount_strong.wlz

8959_wholemount_moderate.wlz

8959_wholemount_weak.wlz

8959_wholemount_possible.wlz

8959_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8959_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon floor plate

strong

regional

strong expression: see section 11 moderate expression: see section 12 weak expression: see section 10

diencephalon roof plate

strong

regional

strong expression: see section 11

metencephalon floor plate

strong

regional

strong expression: see section 11 moderate expression: see section 12 weak expression: see section 08 09 10 13

midbrain floor plate

strong

regional

strong expression: see section 11 moderate expression: see section 12 weak expression: see section 10

spinal cord floor plate

weak

regional

weak expression: see section 11

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 06 16 weak expression: see section 05 08 14 15

tongue muscle

weak

regional

weak expression: see section 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38682
Entity Detected:	Cp, ceruloplasmin ( MGI:88476)
Sequence:	sense strand is shown >T38682 TCGTGAGTACACAGATGGTTCCTTCACAAACCGAAAACAAAGAGGCCCTGATGAGGAACATCTTGGAATC CTAGGTCCTGTCATTTGGGCAGAAGTAGGAGACACCATTAAAGTCACCTTTCATAACAAAGGACAGCATC CTCTCAGCATTCAGCCAATGGGAGTAAGTTTCACTGCAGAAAATGAGGGAACATACTATGGCCCACCAGG TCGCTCCTCACAGCAAGCAGCCTCCCATGTGGCTCCCAAAGAAACCTTTACATACGAATGGACTGTCCCC AAAGAAATGGGACCCACTTATGCAGATCCTGTGTGCCTATCTAAGATGTACTACTCTGGCGTTGACCCCA CCAAAGATATATTTACTGGGCTTATTGGGCCAATGAAAATATGCAAGAAAGGCAGCTTACTTGCTGATGG GAGACAGAAAGATGTAGACAAAGAGTTCTACTTGTTTCCCACAGTGTTTGATGAGAATGAGAGTTTACTC TTAGATGATAATATCAGGATGTTCACAACTGCACCTGATCAAGTGGATAAGGAAGATGAAGACTTTCAGG AGTCTAATAAGATGCACTCCATGAATGGGTTCATGTATGGCAATCAGCCTGGCCTCAATATGTGTCTAGG AGAATCCATCGTGTGGTATTTGTTCAGCGCTGGAAATGAGGCTGATGTGCATGGGATATACTTTTCAGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 155397. Forward Primer - name:155397_F_cDNA_TC1570807, sequence:TCGTGAGTACACAGATGGTTCC; Reverse Primer - name:155397_N_SP6_cDNA_TC1570807, sequence:CCTGAAAAGTATATCCCATGCAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8958 same embryo
	EMAGE:8962 same embryo
	EMAGE:8960 same embryo
	EMAGE:8961 same embryo
	EMAGE:8963 same embryo
	EurExpress:euxassay_012898 same experiment
	MGI:4824021 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source