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EMAGE:896

Lfng LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase ( MGI:1095413)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:896 EMAGE:896
Fig 6A Raft et al, 2004 [PMID:15084464] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig 6C Raft et al, 2004 [PMID:15084464] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: three stars
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Find spatially similar expression patterns: Find spatially similar patterns
Notes:
In A, dorsal is at the top of the figure and medial is to the right. This is a transverse section through the wild-type anterior otocyst showing a lateral to medial gradient of Lfng signal, decreasing medially. Image annotations: Arrow in A - extent of detectable signal; Arrowhead in C - posteroventral Lfng signal.
Expression Pattern Description
Spatial Annotation:
EMAGE:896EMAGE:896EMAGE:896Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context moviewholemount mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
896_wholemount_strong_3D_2.wlz
896_wholemount_notDetected_3D_2.wlz
896_voxel_strong_3D_1.wlz
896_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:896_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
otocyst epithelium
detected detected
regionalThere is a medial-lateral intensity gradient and borders of dorsal and medial expression (Fig 6A). Expression is absent from the Tbx1-positive postero-lateral regions (Fig 6C).
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1271025
Entity Detected:Lfng, LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase ( MGI:1095413)
Notes:The probe used in this study by Raft et al, 2004 [PMID:15084464] is indicated as that used by Morsli et al, 1998 [PMID:9547240] ie. pMFR1, which "contains a 2.2 kb insert including the entire coding region of murine Fng (Cohen et al., 1997 [PMID:9207795] ; Johnston et al., 1997 [PMID:9187150] )". The actual sequence (inc. the start and end points) of pMFR1 are not reported in any of these publications.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:129/Sv x C57BL/6J x FVB x SJL
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:unknown
Procedures
General Information
Authors:Raft et al, 2004 [PMID:15084464] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.01067] [ PMID:15084464] Raft S, Nowotschin S, Liao J, Morrow BE 2004 Suppression of neural fate and control of inner ear morphogenesis by Tbx1. Development (131):1801-12
 [ PMID:9547240] Morsli H, Choo D, Ryan A, Johnson R, Wu DK 1998 Development of the mouse inner ear and origin of its sensory organs. J Neurosci (18):3327-35
 [ doi:10.1038/ng0797-283] [ PMID:9207795] Cohen B, Bashirullah A, Dagnino L, Campbell C, Fisher WW, Leow CC, Whiting E, Ryan D, Zinyk D, Boulianne G, Hui CC, Gallie B, Phillips RA, Lipshitz HD, Egan SE 1997 Fringe boundaries coincide with Notch-dependent patterning centres in mammals and alter Notch-dependent development in Drosophila. Nat Genet (16):283-8
 [ PMID:9187150] Johnston SH, Rauskolb C, Wilson R, Prabhakaran B, Irvine KD, Vogt TF 1997 A family of mammalian Fringe genes implicated in boundary determination and the Notch pathway. Development (124):2245-54
Links:MGI:3044710 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI