EMAGE mouse gene expression spatial database

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EMAGE:9038

Tmem130 transmembrane protein 130 ( MGI:3607706)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012940. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012940_01	euxassay_012940_02	euxassay_012940_03	euxassay_012940_04

euxassay_012940_05	euxassay_012940_06	euxassay_012940_07	euxassay_012940_08	euxassay_012940_09

euxassay_012940_10	euxassay_012940_11	euxassay_012940_12	euxassay_012940_13	euxassay_012940_14

euxassay_012940_15	euxassay_012940_16	euxassay_012940_17	euxassay_012940_18	euxassay_012940_19

euxassay_012940_20	euxassay_012940_21	euxassay_012940_22	euxassay_012940_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9038_wholemount_strong.wlz

9038_wholemount_moderate.wlz

9038_wholemount_weak.wlz

9038_wholemount_possible.wlz

9038_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9038_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 18 19 20 21

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 16 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 07 08 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 08 17 19

spinal cord

strong

regional

strong expression: see section 10 11 12 13 14 15 16

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 16 17 18 weak expression: see section 13 14 15

neural retina

moderate

regional

moderate expression: see section 01 02 03 22 23

nasal cavity olfactory epithelium

moderate

spotted

moderate expression: see section 11 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38183
Entity Detected:	Tmem130, transmembrane protein 130 ( MGI:3607706)
Sequence:	sense strand is shown >T38183 TCTTTTAACCTGACCCACGTCTTCCAGGACCCCGGGGACTACTGCTTCATCTTCCGTGCTGAGAATGCTA TCAGCAAGGCTCACCAGTACCACAGGATTCAGGTGTGGCCCTCCAGCTTCCAGCCATTTGTCTTTGCTTT CCCGTGTGCCACGCTAATCACCGTGCTGCTGGTTTTTCTCATGTATATGACTGTGCGGAGCGCTGCAACC CAGAAGGACATCGTGGAGAGCCCAGGGGCCACTGGGCTCAAGTGTTGCTGCCAGGTGTGCTGTGGATCCC TCTTTCTGGACTCCCCGTCTGAGTACCTGGAAATCGTCCGAGAGAACCACGGGCTGCTTCCACCCCTCTA CAAACCTGTGAAAACTTACAATGTGTGAACTCCCATCCCATACCTCTCAGTATTCACCGATCACCCATCG GGAGCTTCGGGCAGGGTGGCCTATGCTACTGAGCAGGAGGGGTTTACACCGGGGTGACCGTTTGCCCAGA CGGGTCACCGATCTGTACAGTCCAGCTGACATCATTCGTCCTTTGTGTCGCCTCCCCACTGTCATCTACT CATCCGTACAGTCTAGTCAGTGCTGTAAGCTGTTCCCAGTCACCCTCCCCCACCCCCTCTACCAAGCAGG ATTCTGATCTTTGGTGTGTTGTGTTAAGACTCTCCCAAGTGGGTCTGGCTGCCCCTTGCCCATTGCTCAC TGGGGACTACTGAGGTCCCTGGCAATGCAGCTCCGCCCAAGCCAGAAGGAACGAGGAAGGAGGTCACAGA TGGTTAAGGACAGAT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 105877. Forward Primer - name:105877_F_cDNA_C130036G08, sequence:TCTTTTAACCTGACCCACGTCT; Reverse Primer - name:105877_N_SP6_cDNA_C130036G08, sequence:ATCTGTCCTTAACCATCTGTGAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9036 same embryo
	EMAGE:9037 same embryo
	EurExpress:euxassay_012940 same experiment
	MGI:4828769 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source