Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:9045

Neurod4 neurogenic differentiation 4 ( MGI:108055)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9045 EMAGE:9045 EMAGE:9045 EMAGE:9045 EMAGE:9045
"Pseudo-wholemount" of euxassay_019457. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019457_01 euxassay_019457_02 euxassay_019457_03 euxassay_019457_04
EMAGE:9045 EMAGE:9045 EMAGE:9045 EMAGE:9045 EMAGE:9045
euxassay_019457_05 euxassay_019457_06 euxassay_019457_07 euxassay_019457_08 euxassay_019457_09
EMAGE:9045 EMAGE:9045 EMAGE:9045 EMAGE:9045 EMAGE:9045
euxassay_019457_10 euxassay_019457_11 euxassay_019457_12 euxassay_019457_13 euxassay_019457_14
EMAGE:9045 EMAGE:9045 EMAGE:9045 EMAGE:9045 EMAGE:9045
euxassay_019457_15 euxassay_019457_16 euxassay_019457_17 euxassay_019457_18 euxassay_019457_19
EMAGE:9045
euxassay_019457_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9045Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9045_wholemount_strong.wlz
9045_wholemount_moderate.wlz
9045_wholemount_weak.wlz
9045_wholemount_possible.wlz
9045_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9045_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
pituitary gland
strong strong
regionalstrong expression: see section 08 09 10 13 14 15 moderate expression: see section 11 12
pineal primordium
strong strong
regionalstrong expression: see section 13 moderate expression: see section 12
diencephalon lateral wall ventricular layer
strong strong
regionalstrong expression: see section 11 13 moderate expression: see section 12
olfactory cortex ventricular layer
strong strong
regionalstrong expression: see section 08 09 10 13 14 15 16
telencephalon ventricular layer
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 moderate expression: see section 02 18 20 weak expression: see section 01 17 19
medulla oblongata basal plate ventricular layer
moderate moderate
regionalmoderate expression: see section 10 weak expression: see section 12
pons ventricular layer
moderate moderate
regionalmoderate expression: see section 09 weak expression: see section 08
midbrain ventricular layer
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 13 14 15 16 moderate expression: see section 17 18
intermediate grey horn
strong strong
regionalstrong expression: see section 18 moderate expression: see section 11 15 16 17 weak expression: see section 12 13 14
neural retina
strong strong
regionalstrong expression: see section 01 02 19 20
lung
moderate moderate
regionalmoderate expression: see section 05 06 09 10 14 15 16 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55120
Entity Detected:Neurod4, neurogenic differentiation 4 ( MGI:108055)
Sequence:sense strand is shown

>T55120
ATGGCAAAAATGTATATGAAATCCAAGGACATGGTGGAGCTGGTCAACACACAATCCTGGATGGACAAAG
GTCTGAGCTCTCAAAATGAGATGAAGGAGCAAGAGAGAAGACCGGGCTCTTATGGAATGCTCGGAACCTT
AACTGAAGAGCATGACAGTATTGAGGAGGATGAAGAAGAGGAAGAAGATGGAGATAAACCTAAAAGAAGA
GGTCCCAAGAAAAAGAAGATGACTAAAGCTCGCCTTGAAAGATTCAGGGCTCGAAGAGTCAAGGCCAATG
CTAGAGAACGGACCCGGATGCATGGCCTGAATGATGCCTTGGATAATCTTAGGAGAGTCATGCCATGTTA
CTCTAAAACTCAAAAGCTTTCCAAGATAGAGACTCTTCGACTGGCAAGGAACTACATCTGGGCCTTGTCT
GAAGTCCTGGAGACTGGTCAGACACTTGAAGGGAAGGGATTTGTAGAGATGCTATGTAAAGGTCTCTCTC
AACCCACAAGCAACCTGGTTGCTGGATGCCTCCAACTGGGGCCTCAATCTACCCTCCTGGAGAAGCATGA
GGAAAAATCTTCAATTTGTGACTCTACTATCTCTGTCCACAGCTTCAACTATCAGTCTCCAGGGCTCCCC
AGCCCTCCTTATGGCCATATGGAAACACATTCTCTCCATCTCAAGCCTCAACCATTTAAGAGTTTGGGTG
ACTCTTTTGGGAGCCATCCACCTGACTGCAGTACCCCCCCTTATGAGGGTCCACTCACACCACCCCTGAG
CATTAGTGGCAACTTCTCCTTAAAGCAAGACGGCTCCCCTGATTTGGAAAAATCCTACAATTTCATGCCA
CATTATACCTCTGCAAGTCTAAGTTCAGGGCATGTGCATTCAACTCCCTTTCAGACTGGCACTCCCCGCT
ATGATGTTCCTGTAGACCTGAGCTATGATTCCTACTCCCACCATAGCATTGGAACTCAGCTCAATACGAT
CTTCTCTGATTAG
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:ATGGCAAAAATGTATATGAAA; Reverse Primer - name:unspecified, sequence:CTAATCAGAGAAGATCGTATT. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:MGI:4826674 same experiment
 EurExpress:euxassay_019457 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS