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EMAGE:9046

Slc25a3 solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 3 ( MGI:1353498)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9046 EMAGE:9046 EMAGE:9046 EMAGE:9046 EMAGE:9046
"Pseudo-wholemount" of euxassay_017857. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017857_01 euxassay_017857_02 euxassay_017857_03 euxassay_017857_04
EMAGE:9046 EMAGE:9046 EMAGE:9046 EMAGE:9046 EMAGE:9046
euxassay_017857_05 euxassay_017857_06 euxassay_017857_07 euxassay_017857_08 euxassay_017857_09
EMAGE:9046 EMAGE:9046 EMAGE:9046 EMAGE:9046 EMAGE:9046
euxassay_017857_10 euxassay_017857_11 euxassay_017857_12 euxassay_017857_13 euxassay_017857_14
EMAGE:9046 EMAGE:9046 EMAGE:9046 EMAGE:9046 EMAGE:9046
euxassay_017857_15 euxassay_017857_16 euxassay_017857_17 euxassay_017857_18 euxassay_017857_19
EMAGE:9046
euxassay_017857_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9046Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9046_wholemount_strong.wlz
9046_wholemount_moderate.wlz
9046_wholemount_weak.wlz
9046_wholemount_possible.wlz
9046_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9046_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T51013
Entity Detected:Slc25a3, solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 3 ( MGI:1353498)
Sequence:sense strand is shown

>T51013
GGACCCTCAGAAGTACAAAGGCATATTTAATGGATTCTCCATTACACTGAAAGAAGATGGCGTTCGTGGT
TTGGCTAAAGGATGGGCCCCAACTTTGATTGGCTATTCCATGCAAGGGCTCTGCAAATTCGGCTTTTATG
AAGTCTTCAAAGCCTTATATAGCAACATACTTGGTGAGGAAAACACCTACCTGTGGCGCACATCACTGTA
TTTAGCTTCTTCTGCCAGTGCTGAATTCTTCGCTGACATTGCCCTGGCTCCTATGGAAGCTGCTAAAGTT
CGAATTCAAACCCAGCCTGGTTATGCCAACACCTTGAGGGAAGCTGTTCCCAAAATGTATAAAGAGGAAG
GCTTAAATGCGTTCTACAAGGGCGTTGCTCCTCTGTGGATGAGACAGATCCCGTACACCATGATGAAGTT
CGCCTGCTTTGAACGTACTGTTGAAGCTTTGTACAAATTTGTGGTTCCTAAGCCCCGAAGTGAATGTACA
AAGGCAGAGCAGCTGGTTGTGACATTTGTGGCAGGTTACATAGCTGGAGTCTTCTGTGCGATCGTCTCCC
ACCCTGCTGACTCTGTGGTCTCTGTGCTGAATAA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:unspecified; Reverse Primer - name:unspecified, sequence:unspecified. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_017857 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS