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EMAGE:9088

Pld1 phospholipase D1 ( MGI:109585)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9088 EMAGE:9088 EMAGE:9088 EMAGE:9088 EMAGE:9088
"Pseudo-wholemount" of euxassay_018686. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018686_01 euxassay_018686_02 euxassay_018686_03 euxassay_018686_04
EMAGE:9088 EMAGE:9088 EMAGE:9088 EMAGE:9088 EMAGE:9088
euxassay_018686_05 euxassay_018686_06 euxassay_018686_07 euxassay_018686_08 euxassay_018686_09
EMAGE:9088 EMAGE:9088 EMAGE:9088 EMAGE:9088 EMAGE:9088
euxassay_018686_10 euxassay_018686_11 euxassay_018686_12 euxassay_018686_13 euxassay_018686_14
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euxassay_018686_15 euxassay_018686_16 euxassay_018686_17 euxassay_018686_18 euxassay_018686_19
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euxassay_018686_20 euxassay_018686_21 euxassay_018686_22 euxassay_018686_23 euxassay_018686_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9088Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9088_wholemount_strong.wlz
9088_wholemount_moderate.wlz
9088_wholemount_weak.wlz
9088_wholemount_possible.wlz
9088_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9088_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50845
Entity Detected:Pld1, phospholipase D1 ( MGI:109585)
Sequence:sense strand is shown

>T50845
TTGGAGAGCTGCTTTGATGGTCCTCAAGGATCTAGATGATGCCTGTACCCAACCTGATCCTAATGTGAGC
ATATCTACTAAGCCAAGACATTGTTCTTTGTTCTTTTTCAGTCACTGTAGGGACAGAACAGGCTTTGCCT
ACCTAACTACATACATTAAGCACTTCTAAGGACATCAATACTGTATCTTAATAACAGGAAGGCATTAAAT
CCTAATACTGAAGAAGGATTTCCCTCTGTTCCCAAAGATACTGCCCAGCTGTGTCCTTGTCATCTGATAA
CATTTGTCAACATGTGCAGCATATGTCAAAACATTGTAACCAGATACGCACAGATGGGACCTCCAAGATG
TCAGTGGGAATCCAGTTCAGATAAAGGACAGGATTTCTGTCTGTCTGAAGAATCCATGTCCTCCTTAGCC
ACTGGATTGACAGAACCAAGTTGATTTTCTCCATTATAAGGCCAGTGGATGTAATTTGTCTTTCAAGTGA
ATCAAAGGCTTTACCTTCAGTTTTCCACTATACCTTCTTTAGGGGAAAAGGCAATTGATTATACAAGACC
TCTACTTTGCTTGAACTGTTAACCATGTTGTATCATTCCAAGACAGCTCCCAAAGAAGCTGAAACACTAA
CTCACATTGTTCCGATCTGAAATGTGGGTATCCACGCTCCCTCTCAGCTGGTATTATGCCCTTTGTAGAC
CAAATTTCAGAGCTCCTCTTGCATCCTGGGAGGGTCTACAGGTTCCTGGAAGCCTGGGACTTGTGCATAC
GAGGTACCTATGTGATTGTAAACAAAAGGGATTGCTGGAGTAAAATTTCCTTTAAGGTGCTCTTCAAAGC
AACTCCCAACCCAT
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TTGGAGAGCTGCTTTGATGG; Reverse Primer - name:unspecified, sequence:ATGGGTTGGGAGTTGCTTTG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9090 same embryo
 EMAGE:9087 same embryo
 EMAGE:9089 same embryo
 EMAGE:9086 same embryo
 EurExpress:euxassay_018686 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS