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EMAGE:9091

Mapkbp1 mitogen-activated protein kinase binding protein 1 ( MGI:1347004)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9091 EMAGE:9091 EMAGE:9091 EMAGE:9091 EMAGE:9091
"Pseudo-wholemount" of euxassay_018677. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018677_01 euxassay_018677_02 euxassay_018677_03 euxassay_018677_04
EMAGE:9091 EMAGE:9091 EMAGE:9091 EMAGE:9091 EMAGE:9091
euxassay_018677_05 euxassay_018677_06 euxassay_018677_07 euxassay_018677_08 euxassay_018677_09
EMAGE:9091 EMAGE:9091 EMAGE:9091 EMAGE:9091 EMAGE:9091
euxassay_018677_10 euxassay_018677_11 euxassay_018677_12 euxassay_018677_13 euxassay_018677_14
EMAGE:9091 EMAGE:9091 EMAGE:9091 EMAGE:9091 EMAGE:9091
euxassay_018677_15 euxassay_018677_16 euxassay_018677_17 euxassay_018677_18 euxassay_018677_19
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euxassay_018677_20 euxassay_018677_21 euxassay_018677_22 euxassay_018677_23 euxassay_018677_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9091Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9091_wholemount_strong.wlz
9091_wholemount_moderate.wlz
9091_wholemount_weak.wlz
9091_wholemount_possible.wlz
9091_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9091_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50666
Entity Detected:Mapkbp1, mitogen-activated protein kinase binding protein 1 ( MGI:1347004)
Sequence:sense strand is shown

>T50666
TCTGGTGAGCAGCTGAAAGGCAGTGGTGCCACTCCTCCAGGAGCACCCCCAGAAATGGAACCCTCTTCTG
GCAACTCTGGCCCCAAGCAGGTGGCTCCTGTGCTGTTGACACGACGGCGTAACAACTTGGACAACAGCTG
GGCCTCCAAGAAAATGGCTGCAACCCGGCCTTTAGCTGGACTCCAGAAAGCCCAGTCTGTGCATAGTTTG
GTACCACAGGATGAGGTGCCTTCATCACGTCCACTGCTCTTCCGGGAGGCAGAGACCCAGGGCAGCTTAG
GATCCCTGCCACAAGCTGGTGGCTGCTCATCTCAGCCCCACTCCTACCAGAACCACACCACCAGTTCTAT
GGCCAAGCTAGCGCGTAGTATTTCTGTTGGCGAGAATCCGGGCCTGGCAACTGAACCTCAAGCTCCTGCA
CCGATCCGAATCTCACCATTCAACAAACTAGCTCTGCCTAGCAGGGCTCACCTTGTCCTGGACATCCCCA
AACCACTTCCTGACCGTCCTACTCTGACCACATTCTCACCTGTATCCAAGGGCCTGACCCACAATGAAAC
AGAACAATCGGGGCCCCTTCGTGAGCCTAGGAAGGCTCATACTACAGTTGAAAAGCACTCCTGTTTAGGG
GAGGGTACTACTCATAAATCTAGGACAGAGTGCCAGGCTTATCCTGGACCCAACCACCCCTGTCGCCAGC
AACTGCCAGTCAACAACCTTCTCCAAGCTGAGAGCTTGCAGCCCCTGTCCCCTGAGAAGACTCGTAACCC
CGTGGAAAGCAGCAGGCCAGGGGTAGCCCTGAGCCAGGACTCAGAACTGGCCTTGAGTCTGCAACAGTGT
GAACAGCTCGTGGCAGAGCTCCAGGGGAATGTACGCCAGGCAGTGGAGCTCTACCGCGCGGTGACCAGCT
GTAAGACACCTTCGGCAGAGCAAAGTCACATCACCCGTCT
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TCTGGTGAGCAGCTGAAAGG; Reverse Primer - name:unspecified, sequence:AGACGGGTGATGTGACTTTG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9093 same embryo
 EMAGE:9092 same embryo
 EMAGE:9094 same embryo
 EurExpress:euxassay_018677 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS