Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:91

Otx2 orthodenticle homolog 2 (Drosophila) ( MGI:97451)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:91
Figure 4.K, Theil et al., 1999 [PMID:10409502] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:91Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
91_wholemount_strong_3D_1.wlz
91_wholemount_moderate_3D_1.wlz
91_wholemount_possible_3D_1.wlz
91_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:91_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
forebrain
detected detected
regional
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1331375
Entity Detected:Otx2, orthodenticle homolog 2 (Drosophila) ( MGI:97451)
Sequence:sense strand is shown

>MGI:1331375
GCCAGTTCAGTCCCCCCTCTAGTACCTCAGTCCCAACCATTGCCAGCAGCAGTGCTCCAGTGTCTATCTG
GAGCCCAGCGTCCATCTCCCCACTGTCTGACCCCTTGTCCACTTCCTCCTCCTGCATGCAGAGGTCCTAT
CCCATGACCTATACTCAGGCTTCAGGTTATAGTCAAGGCTATGCTGGCTCAACTTCCTACTTTGGGGGCA
TGGACTGTGGATCTTATTTGACCCCTATGCATCACCAGCTTCCTGGACCAGGGG
nt 718 - nt 981 of NM_144841.1
Notes:The Otx2 probe used in this study by Theil et al., 1999 [PMID:10409502] is indicated as being originally described by Simeone et al., 1993 [PMID:8101484] ie. as "a 263 nucleotide HaeIII fragment". See also Figure 1a of Simeone et al. for diagram. This probe is found within the coding region, 3' to the homeodomain coding region.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Strain:C57BL/6 x C3H
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Theil et al., 1999 [PMID:10409502] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10409502] Theil T, Alvarez-Bolado G, Walter A, Ruther U 1999 Gli3 is required for Emx gene expression during dorsal telencephalon development. Development (126):3561-71
 [ PMID:8101484] Simeone A, Acampora D, Mallamaci A, Stornaiuolo A, D'Apice MR, Nigro V, Boncinelli E 1993 A vertebrate gene related to orthodenticle contains a homeodomain of the bicoid class and demarcates anterior neuroectoderm in the gastrulating mouse embryo. EMBO J (12):2735-47
Links:MGI:1340418 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI